PowerPoint me in The right direction in Lab

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(Power)Point me in The right direction in Lab
Mark Walvoord University of Oklahoma ABLE 2008
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Chicken Wing Microbiology A lesson in food
safety and microbioogical technique

• Mariëlle Hoefnagels
• Mark Walvoord

ABLE 2005 Proceedings, Vol. 27185-200
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• Two main objectives
• Measure populations of bacteria on chicken wings
• Design an experiment to test a treatment that
  might get rid of the bacteria
• Important lessons
• Basic microbiology techniques serial dilution,
  plate count, aseptic technique
• Food safety
• Experimental design (investigative)

Aseptic Technique Overall goal Avoid
contaminating your solutions or anything sterile,
including pipets, saline, and agar. For example
Wash your hands before you begin ( afterwards)
Wipe down bench with Lysol before you begin
Discard any plates with existing colonies Open
containers/petri dishes only when necessary, and
then only briefly Never touch pipet tip to any
surface except the solution you are pipetting
Use each pipet only once, then set aside

• Timeline
• Week 1 set up initial counts of bacteria on
  chicken wings
• Week 2 count bacteria from week 1 design and
  implement independent experiments
• Week 3 collect data from independent
  experiments write report to Wing Nut Chicken
  Wing Co.

Procedures

• Label your plates test tubes
• Make wing washes for two chicken wings (per
  group)
• Make serial dilution (through 10-7 in test tubes)
  for each wing
• Inoculate agar plates (2 duplicate plates per
  tube)
• Clean UP
• After, put contaminated trash (gloves, chicken,
  used pipets) in autoclave bags
• Put clean trash (pipet wrappers, paper towels,
  etc.) in rooms trashcan

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