(1) This year

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(1) This years ERP program (2) A Short Talk
About A Short Talk (3) Fetal origins of
polycystic ovary syndrome David H Abbott,
Ph.D. Department of Ob/Gyn National Primate
Research Center ERP Training Program
University of Wisconsin-Madison ERP Seminar
Series - Opening Seminar Fall Semester
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ERP Fall 2005 seminars Alternate weeks - ERP
students - invited faculty
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ERP Fall 2005 seminars Alternate weeks - ERP
students - invited faculty ERP students
20-min talks ( 5-min questions) will
progress from - 1st year, outline of project
(voluntary), to - 2nd years, project
development (e.g. National Meeting
presentation), to - last year, thesis defense (
45-min talk, ?? min questions)
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ERP Fall 2005 seminars Alternate weeks - ERP
students - invited faculty ERP students
20-min talks ( 5-min questions) will
progress from - 1st year, outline of project
(voluntary), to - 2nd years, project
development (e.g. National Meeting
presentation) - last year, thesis defense (
45-min talk, ?? min questions) Invited
faculty - chosen from suggestions made by ERP
students and faculty - will provide high
quality talks and science to which students
should aspire
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ERP Fall 2005 seminars ERP is an
Inter-Departmental Program ERP seminar -
provides a weekly focus for the program -
communicates scientific developments achieved
by students and faculty in the program -
permits communication and discussion of local or
national ERP program events
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ERP Fall 2005 Symposium November 21st, 2005
ERP students - poster presentations are
required by all - 1st year students are
encouraged to give some form of outline of their
project as a poster, at a minimum - some of
the student presentations will be selected for
talks by the Symposium Committee
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ERP Seminar - student talks
In preparing your talk remember. - priority
of attention in your audience is 70 how you
look, act and appear 20 how you sound
10 what you say
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President John F. Kennedys famous speech near
the Berlin Wall, Germany in 1963
Ich bin ein Berliner I am a Berliner
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President John F. Kennedys famous speech near
the Berlin Wall, Germany in 1963
I am a Berliner Ich bin ein Berliner I am
a doughnut
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President John F. Kennedys famous speech near
the Berlin Wall, Germany in 1963
Ich bin Berliner I am a Berliner Ich bin
ein Berliner I am a doughnut
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President John F. Kennedys famous speech near
the Berlin Wall, Germany in 1963
Ich bin ein Berliner I am one with the
people of Berlin
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ERP Seminar - student talks
In science as in love, too much concentration
on technique can often lead to
impotence. P.L. Berger Sociologist and author
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ERP Seminar - student talks
Keep It Simple Stupid
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ERP Seminar - student talks
The hallmark of any successful scientific talk is
clarity. To achieve clarity, the talk must be
well organized and logically structured. A talk
needs an ? introduction ? methods ?
results ? and a summary/conclusion
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ERP Seminar - student talks
The hallmark of any successful scientific talk is
clarity. To achieve clarity, the talk must be
well organized and logically structured. A talk
needs an State your points THREE times ?
introduction ? methods ? results ? and a
summary/conclusion
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ERP Seminar - student talks
The hallmark of any successful scientific talk is
clarity. To achieve clarity, the talk must be
well organized and logically structured. A talk
needs an State your points THREE times ?
introduction (1) say what you are going to
say ? methods ? results ? and a
summary/conclusion
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ERP Seminar - student talks
The hallmark of any successful scientific talk is
clarity. To achieve clarity, the talk must be
well organized and logically structured. A talk
needs an State your points THREE times ?
introduction (1) say what you are going to
say ? methods.. (2) say it ?
results. ? and a summary/conclusion
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ERP Seminar - student talks
The hallmark of any successful scientific talk is
clarity. To achieve clarity, the talk must be
well organized and logically structured. A talk
needs an State your points THREE times ?
introduction (1) say what you are going to
say ? methods.. (2) say it ?
results. ? and a summary/conclusion. (3)
say you have said it
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ERP Seminar - student talks

• For a 20-minute ERP student talk -
• ? Introduction
• - statement of the problem/hypothesis
• - include your motivation to solve the
  problem/hypothesis,
• e.g. clinical pathology
• ? maximum of 3-4 slides

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ERP Seminar - student talks

• For a 20-minute ERP student talk -
• ? Methods
• - experimental design
• - approaches/techniques used
• - caveats, e.g. data outlier
• ? maximum of 3-4 slides

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ERP Seminar - student talks

• For a 20-minute ERP student talk -
• ? Results
• - brief, clear summary of your MAIN
  findings
• - less important results will emerge
  during questions
• ? maximum of 5-8 slides

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ERP Seminar - student talks

• For a 20-minute ERP student talk -
• ? Summary/Conclusion
• - condensed summary of your findings
• ( 3-4 points)
• - relevance of your findings to your
  problem/hypothesis in the Introduction
• ? maximum of 2-3 slides

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ERP Seminar - student talks

• For a 20-minute ERP student talk -
• ? Total of 13-19 slides
• Use at least 18pt size font, Helvetica
• Keep the results diagrams or tables easy to
  understand
• Acknowledge those who helped you, sources of
  funding, etc.
• ? Practice, practice, practice

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ERP Seminar - student talks

• Slides from this talk
• and a more detailed handout
• will be posted at either
• Learn_at_UW or on the ERP website.

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Fetal origins of polycystic ovary syndrome

• University of Wisconsin, Madison,WI Reproductive
  Medicine and Infertility Associates, MN
• Deborah Barnett, Ph.D. Daniel Dumesic, M.D.
• Cristin Bruns, M.D.
• Dee Schramm, Ph.D.
• Ricki Colman, Ph.D.
• Joseph Kemnitz, Ph.D.
• Theodore Goodfriend, M.D. University of
  Michigan, Ann Arbor, MI
• Rao Zhou, M.D. Vasantha Padmanabhan, Ph.D.
• Beverly Hutcherson, B.S.
• Sarah Hoffmann University of Edinburgh, UK
• Karla Meinholz, B.S. Alan McNeilly, Ph.D.,
  D.Sc.
• Scott Medlin, B.S.
• Scott Muller, B.S. University of London, UK
• Brynn Hogan, B.S. Stephen Franks, M.D.
• Nora Shumway
• Kurt Sladky, D.V.M. Cato Research, NC
• Joel Eisner, Ph.D.
• University of California, Davis, CA

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• obesity

33 of women (35 million, USA)

• type 2 diabetes

8 of women (8.2 million, USA)

• anovulatory infertility

27 of infertility cases (USA and W. Europe)
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• obesity

Polycystic ovary syndrome (PCOS)

• type 2 diabetes

• anovulatory infertility

5-10 women of reproductive age (and their
male close relatives)
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Important Reproductive, Metabolic and General
Health Disorders that are Manifest in Women with
PCOS
(a) Reproductive disorders Hyperandrogenism
(ovarian and adrenal hirsutism) Amenorrhea
(intermittent or complete) Polycystic
ovaries High rates of miscarriage (IVF
cycles) Hypersecretion of LH (b) Metabolic
disorders Hyperinsulinemia (insulin resistance
and type 2 diabetes) Impaired pancreatic ß-cell
insulin secretion Obesity (including
preferential abdominal adiposity) Hyperlipidemia
(c) General health disorders Cardiovascular
disease Endometrial cancer Sleep apnea .but
the etiology of PCOS remains unknown.
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Abbott et al (2002) J Endocr. 1741-5
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Discrete Prenatal Androgen Excess During Gestation
(10-15 mg testosterone propionate injected s.c.
daily into pregnant mothers)
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Serum testosterone levels

P lt 0.001 vs controls

(a) Mothers
Oil injected controls (n5)

TP injected females (n9)
Backtransformed means ?95 CI
(b) Fetuses
(c) Infants
TP injected

TP injected mothers
Oil injected
Oil injected mothers
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Ovulatory menstrual cycles in control and
prenatally androgenized females over a 6-month
period

p lt 0.02 vs. controls

p lt 0.03 vs. controls
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Increased Serum Testosterone Levels in Prenatally
Androgenized Females (an i.m. injection of 200 IU
rhCG was given at 0h)


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Serum Levels of (a) immuno-LH in 13-14 year old
Females during the Follicular Phase and (b)
bioactive LH in 18-20 year old Females during
Ovarian Stimulation with rhFSH

(a)
(b)

p lt 0.001 vs controls
p lt 0.03 vs controls
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Hypothesis for Prenatal Androgen Programming of
Females for PCOS


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Hypothesis for Prenatal Androgen Programming of
Females for PCOS