Engineering New Organisms

1
Engineering New Organisms
2
Genetic Engineering

• Cutting and splicing of DNA from different
  sources
• Genes can be inserted into almost any organism
• Transgenic organisms
• An organism that has been transformed with genes
  from another organism
•  Usually done to increase the production of
  valuable proteins

3
More on Human Genetics

• Diseases and CSI (not the learning center one)

4
Restriction Fragment Length Polymorphism (RFLP)

• Isolation of a specific nucleotide sequence
• Digestion of DNA with restriction enzymes
• Separate the pieces on an agarose gel
• Probe the separated sequences with labeled probe
  (complimentary to the separated pieces)
  nucleotides

5
RFLP

• RFLPs are inherited
• Different people have different sets of RFLPs
• Thus an individuals DNA can be identified
•  RFLPs can be used as for diagnosis of genetic
  disease
• RFLPs can be used for DNA fingerprinting

6
Human Genome

• Sequence the entire set of human chromosomes
• Handout supplement from DOE website on human
  genome
• Highlights of the human genome project