Title: BD BACTEC 9050 Blood Culture system
1BD BACTEC 9050Blood Culture system
2Clinical Importance of Blood Cultures
- The earliest possible identification of
bloodstream infections allows for - Prompt optimization of antimicrobial therapy
- Diminished need for additional diagnostic testing
- Decreased length of hospital stay
- Decreased healthcare costs
- Increased survival rates
- However due to its clinical importance, every
hour of earlier detection is crucial Vigano et
al, Diag. Micro. Inf.Dis.., 44 (2002)
3Introduction
- Rapid detection of bacteria fungi.
- Samples drawn from patients and injected
directly into BACTEC culture vials. - Vials entered into instrument as soon as
possible for maximum performance efficiency.
4Principle of Test
5Media
6Media
- Standard Aerobic/F
- Enriched Soybean-Casein Digest broth.
- Added CO2
- Aerobic microorganisms (bacteria and yeasts)
- Allows for 10ml of blood.
- No resins
7Media
- Standard Anaerobic/F
- Prereduced enriched Soybean-Casein digest broth
- Added CO2
- Recovery of anaerobic organisms
- 10ml Blood volume
- No resins
8Media
- Peds Plus/F
- Aerobic bacteria and yeasts
- Resins for increased recovery
- Pediatric or immunocompromised patients
- 3 5ml blood volume
9Resin Media
- Non-ionic and cationic exchangers
- Neutralizes most antibiotics
- Helps to lyse blood cells so that intra-celllar
organisms are set free - Provide the organsims with growth-centres to
enhance speed and recovery rate.
10Blood CollectionSpecimen identification
Label both vials with patient demographics
11VERY IMPORTANT
DO NOT WRITE ON THE BARCODE LABEL
12Identify the test request
Remove the tear-off bar code strip and stick it
on the test request form
13Preparation of the vials
Remove the flip-off cap and disinfect the septum
with 70 Isopropanol or Ethanol
14Selection of the puncture site
Apply the tourniquet and select the vein
15Decontaminate the puncture site
Disinfect circularly from the inside to the
outside with povidone/iodine for 60 seconds
16Blood volume and inoculation sequence
- Inoculation Volume
- Mark the desired volume on the vials label
before inoculation - Adults 8 10ml
- Peds 1 3ml
- Myco/F 3 5ml
- Inoculation sequence
- Aerobic
- Anaerobic
17Mixing and transportation of the vials
Gently tip over several times
Bring to the lab as soon as possible
18Delayed Vial Entry
- Plus Aerobic/F Plus Anaerobic/F Peds Plus/F and
Lytic/10 Anaerobic/F media - 20 hours pre incubation _at_ 35C
- 48 hours _at_ room temperature
- Standard Aerobic and Standard Anaerobic media
- 12 hours pre incubation _at_ 35C
- 48 hours _at_ room temperature
19Ease of Use
50 Vial capacity
Operator instructions
LCD and keypad
Volume adjustable alarm
Fixed barcode reader with easy alignment
Built in temperature compensation std
20Functions
- There are three main functions
- Vial loading
- Remove negatives
- Remove positives
21- Vials may be held in the instrument for 5 7
days - Positive cultures may be detected with in 24
hours. - All positive cultures must be sub cultured and
gram stained.
22Notification of positive vials
- New positive indicator lamp on the front of the
instrument flashes. - An audible alarm sounds
- On the main status display, the station is
displayed as a filled circle with a plus ()
sign. - On opening the door the following sign appears
23Automated vs Manual Blood cultureClinical study,
1442 blood culture sets. 16,4 of the samples
were positive.
- Cumulative Detection Rate ()
Time (hours)
Saleh, A.F. et al. 8th ECCMID 1997
24In summary
- The gold standard for definitive diagnosis of
septicemia and fungemia. - A simple, direct method which can be implemented
quickly. - Safer no venting and non invasive.
- DVE
- Faster time to detection.
- Storage at room temperature.
- No additional sample preparation or enrichment.
25Thank you!