BD BACTEC 9050 Blood Culture system - PowerPoint PPT Presentation

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BD BACTEC 9050 Blood Culture system

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Vials entered into instrument as soon as possible for ... Delayed Vial Entry. Plus Aerobic/F; Plus Anaerobic/F; Peds Plus/F and Lytic/10 Anaerobic/F media ... – PowerPoint PPT presentation

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Title: BD BACTEC 9050 Blood Culture system


1
BD BACTEC 9050Blood Culture system
2
Clinical Importance of Blood Cultures
  • The earliest possible identification of
    bloodstream infections allows for
  • Prompt optimization of antimicrobial therapy
  • Diminished need for additional diagnostic testing
  • Decreased length of hospital stay
  • Decreased healthcare costs
  • Increased survival rates
  • However due to its clinical importance, every
    hour of earlier detection is crucial Vigano et
    al, Diag. Micro. Inf.Dis.., 44 (2002)

3
Introduction
  • Rapid detection of bacteria fungi.
  • Samples drawn from patients and injected
    directly into BACTEC culture vials.
  • Vials entered into instrument as soon as
    possible for maximum performance efficiency.

4
Principle of Test
5
Media
6
Media
  • Standard Aerobic/F
  • Enriched Soybean-Casein Digest broth.
  • Added CO2
  • Aerobic microorganisms (bacteria and yeasts)
  • Allows for 10ml of blood.
  • No resins

7
Media
  • Standard Anaerobic/F
  • Prereduced enriched Soybean-Casein digest broth
  • Added CO2
  • Recovery of anaerobic organisms
  • 10ml Blood volume
  • No resins

8
Media
  • Peds Plus/F
  • Aerobic bacteria and yeasts
  • Resins for increased recovery
  • Pediatric or immunocompromised patients
  • 3 5ml blood volume

9
Resin Media
  • Non-ionic and cationic exchangers
  • Neutralizes most antibiotics
  • Helps to lyse blood cells so that intra-celllar
    organisms are set free
  • Provide the organsims with growth-centres to
    enhance speed and recovery rate.

10
Blood CollectionSpecimen identification
Label both vials with patient demographics
11
VERY IMPORTANT
DO NOT WRITE ON THE BARCODE LABEL
12
Identify the test request
Remove the tear-off bar code strip and stick it
on the test request form
13
Preparation of the vials
Remove the flip-off cap and disinfect the septum
with 70 Isopropanol or Ethanol
14
Selection of the puncture site
Apply the tourniquet and select the vein
15
Decontaminate the puncture site
Disinfect circularly from the inside to the
outside with povidone/iodine for 60 seconds
16
Blood volume and inoculation sequence
  • Inoculation Volume
  • Mark the desired volume on the vials label
    before inoculation
  • Adults 8 10ml
  • Peds 1 3ml
  • Myco/F 3 5ml
  • Inoculation sequence
  • Aerobic
  • Anaerobic

17
Mixing and transportation of the vials
Gently tip over several times
Bring to the lab as soon as possible
18
Delayed Vial Entry
  • Plus Aerobic/F Plus Anaerobic/F Peds Plus/F and
    Lytic/10 Anaerobic/F media
  • 20 hours pre incubation _at_ 35C
  • 48 hours _at_ room temperature
  • Standard Aerobic and Standard Anaerobic media
  • 12 hours pre incubation _at_ 35C
  • 48 hours _at_ room temperature

19
Ease of Use
50 Vial capacity
Operator instructions
LCD and keypad
Volume adjustable alarm
Fixed barcode reader with easy alignment
Built in temperature compensation std
20
Functions
  • There are three main functions
  • Vial loading
  • Remove negatives
  • Remove positives

21
  • Vials may be held in the instrument for 5 7
    days
  • Positive cultures may be detected with in 24
    hours.
  • All positive cultures must be sub cultured and
    gram stained.

22
Notification of positive vials
  • New positive indicator lamp on the front of the
    instrument flashes.
  • An audible alarm sounds
  • On the main status display, the station is
    displayed as a filled circle with a plus ()
    sign.
  • On opening the door the following sign appears

23
Automated vs Manual Blood cultureClinical study,
1442 blood culture sets. 16,4 of the samples
were positive.
  • Cumulative Detection Rate ()


Time (hours)
Saleh, A.F. et al. 8th ECCMID 1997
24
In summary
  • The gold standard for definitive diagnosis of
    septicemia and fungemia.
  • A simple, direct method which can be implemented
    quickly.
  • Safer no venting and non invasive.
  • DVE
  • Faster time to detection.
  • Storage at room temperature.
  • No additional sample preparation or enrichment.

25
Thank you!
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