PhD 9903 - PowerPoint PPT Presentation

1 / 5
About This Presentation
Title:

PhD 9903

Description:

Chlamydia pneumoniae causes respiratory tract diseases, possible involvement in ... The role of the infinitely small in nature is infinitely large Louis Pasteur. PhD ... – PowerPoint PPT presentation

Number of Views:63
Avg rating:3.0/5.0
Slides: 6
Provided by: tinam1
Category:
Tags: phd | louis | pasteur

less

Transcript and Presenter's Notes

Title: PhD 9903


1
PhD 99-03
  • PhD thesis Chlamydia pneumoniae PCR detection
    and cell-mediated immune response to infection.
  • Chlamydia 4 species, 3 human pathogens Among
    the smallest known bacteria (genome 1-1.23 Mbp).
    Obligate intracellular parasite, gram-negative.
  • Chlamydia pneumoniae causes respiratory tract
    diseases, possible involvement in cardiovascular
    disease

The role of the infinitely small in nature is
infinitely large Louis Pasteur
2
PhD
  • Aims of PhD studies
  • To develop a quantitative real-time PCR
    (LightCycler) for C. pneumoniae detection
  • To test the real-time PCR method patient
    samples, conventional PCR methods
  • To test different DNA purification methods
    atherosclerotic tissue
  • To set up an infection/protection model for C.
    pneumoniae in mice
  • To test the usefulness of real-time PCR for
    determination of bacterial load
  • To test whether Pmps are recognised by a
    cell-mediated immune response in mice. Are Pmps
    vaccine candidates?

3
PhD - Methods
  • Cultivation of obligate intracellular bacteria in
    eukaryotic cells.
  • Real-time PCR
  • DNA extraction methods
  • Immunohistochemistry
  • ELISpot assay (detection of T-cell reponses to
    specific antigens)
  • Mouse model of respiratory tract infection

4
PhD - publications
  • 1.     Mygind T, Birkelund S, Falk E,
    Christiansen G. 2001. Evaluation of real-time
    quantitative PCR for identification and
    quantification of Chlamydia pneumoniae by
    comparison with immunohistochemistry. J.
    Microbiol. Methods. 46(3) 241-251.
  • 2.     Mygind, T., Birkelund, S., Christiansen,
    G. 2002. Detection and quantification of
    experimental Chlamydia pneumoniae infection in
    immunized and control mice by real-time PCR and
    by weight loss determination. In Proceedings of
    the Tenth International Symposium on Human
    Chlamydial Infections. Grafmat, Turkey, pp.
    309-312.
  • 3.     Mygind, T., Birkelund, S., Birkebæk, N.H.,
    Østergaard, L., Jensen, J.S., G. Christiansen.
    2002. Determination of PCR efficiency in
    chelex-100 purified clinical samples and
    comparison of real-time quantitative PCR and
    conventional PCR for detection of Chlamydia
    pneumoniae. BMC Microbiology 2 17.
  • 4.     Mygind, T., Østergaard, L., Birkelund, S.,
    Lindholt, J. S., Christiansen, G. 2003.
    Evaluation of five DNA extraction methods for
    purification of DNA from atherosclerotic tissue
    and estimation of prevalence of Chlamydia
    pneumoniae in a Danish population undergoing
    vascular repair. BMC Microbiology 3 19.
  • 5.     Mygind, T., Vandahl, B. B., Pedersen, A.
    S., Höllsberg, P., Birkelund, S., Christiansen,
    G. 2004. Identification of an in vivo CD4 T
    cell-mediated response to polymorphic membrane
    proteins of Chlamydia pneumoniae FEMS Immunology
    and Medical Microbiology. 40(2) 129-137

5
Loke Diagnostics 03
  • Project leader
  • Cloning and production of bacterial antigens for
    serum ELISA
  • Vaccine development C. pneumoniae
  • Large scale cultivation of C. pneumoniae
  • Purification of COMC
  • Contact to German and American collaborators
Write a Comment
User Comments (0)
About PowerShow.com