PBMC

1
FISH EAS FISH
Specificity of EAS FISH
DK(LCL) DK(LCL)
EBER1 is an RNA species encoded by
Epstein-Barr Virus (EBV). Various cell lines
were probed with an antisense probe for EBER1
(filled histograms) or with a nonsense probe for
EBER1 (open histograms). The probes were
detected by fluorescence in situ hybridization
(FISH) or by FISH with enzymatic amplification of
the signal (EAS FISH). DK(LCL) and JY(LCL are
EBV cells. BJAB is a B cell line that is not
transformed by EBV and that thereby does not
express any EBV mRNA. PBMC are peripheral blood
mononuclear cells from a healthy volunteer. The
results demonstrate that EAS FISH demonstrates
significantly greater signal for EBER1 than FISH
does and that the signal seen with EAS FISH is
found only in cells that are transformed by EBV.
JY(LCL) JY(LCL)
EAS FISH EAS FISH
PBMC
BJAB
Log Fluorescence Intensity