11cm, pH710 Biorad ReadyStrip

1
DeStreak Optimization 2
Murine bladder tissue lysate
10
7
50kda
12 T 5 C Single conc. Gel Criterion, 1mm
11cm, pH7-10 Biorad ReadyStrip
IEF 200-400-800-1600V 2h/ea, 8000V 32kVhr
Stain Kangs colloidal Coomassie, Typhoon scan
575V
2
Bladder (crude lysate) proteome map. pH4-7
Murine bladder (crude) tissue lysate
4
7
50kda
12 T 5 C Single conc. Gel Hoefer SE600 1mm
thick 16x16cm gel
17cm, pH4-7 Biorad ReadyStrip, In-gel rehydration
IEF Biorad preset, 65kVhr
Stain Kangs colloidal Coomassie, Typhoon scan
575V