ELFInduced DNA Breaks

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ELF-Induced DNA Breaks

• Henry Lai
• Department of Bioengineering
• University of Washington
• Seattle, WA
• USA

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DNA Strand breaks -single and double strand
breaks -mis-repair cumulative -affect cell
functions can lead to cancer and cell death
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COMET ASSAY
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DNA strand breaks have been reported in different
cell types after ELF EMF exposure. Ahuja (99)-
human leukocytes Delimaris (06)- human
lymphocytes Hong (05)- mouse sperm cells Ivancsit
(02, 05)- human fibroblasts and
melanocytes Ivancsit (03)-more pronounced effect
observed in fibroblasts of older
subjects. Jajte (01)- rat lymphocytes Lai and
Singh (97, 04)- rat brain cells Li (01)- E.
coli Svedenstal (99)- mouse brain cells Wolf
(05)- HL-60 and rat fibroblasts Zmyslong (00,
04)- rat lymphocytes
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ELF EMF affects DNA repair Robison (02) HL-60
decrease Chow (00) E. coli- increase- heat
shock protein Schmitz (04)- Mouse brain and
kidney cells- increase
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ELF EMF genotoxic effects involve free
radicals Effects blocked by free radical
scavengers, enhanced by divalent metals, increase
oxidative-damage in molecules Jaite (01) Lai
and Singh (97) Li (01) Lourencini (00) Yokus
(05) Wolf (05) Zmyslong (00, 04)
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Electromagnetic fields
iron
OH
H2O2
mitochondria
Cellular damage
THE FENTON REACTION
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Intensity and duration of exposure
interact. High intensity/short duration Low
intensity/long duration