Western Blot Apparatus

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Western Blot Apparatus
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We will use premade gels they are difficult to
pour and contain toxic components
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Remember to wear gloves when handling gels
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Remove gel from box and tear side of wrapping
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Remove gel from protective pouch
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You need to pull and remove the strip at the
bottom of the gel
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Remove the comb from the gel(after the apparatus
is set up)and wash out the wells
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Remove the comb from the gel
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Setting up the gel

• Place your gel in the top apparatus to the left
• The wells face in
• After the gel is placed in, put the top apparatus
  into the bottom apparatus close the locks
• You must have 2 gels use a blank gel if you
  have only one gel to run

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Final setup of gels
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Make sure the wells are facing inward
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2 gels should be put into the apparatus

• Buffer can then be poured in between the 2 plates
• Notice the black and red electrodes

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Place the entire gel apparatus into the tank
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You may want to use longer/thinner tips to load
the gel
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Remember, the wells are in the back of the gel

• Make sure you find the wells
• Careful place the tip as far down as possible
• Load the well

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Another picture of gel loading
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The final step

• Put the cover on in the correct orientation
• Red to red and black to black