HORIBA Jobin Yvon

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Fluorometer
                       
fluorescence
HORIBA Jobin Yvon
Instrument presentation feb-15-06
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World's the most sensitive spectrofluorometer
Totally computer-controlled
                               
xenon flash lamps are a convenient source of
ultraviolet (UV) and visible light. These lamps
generate a minimal amount of heat and are
available in sizes ranging from 5 to 60 watts.
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Rad. source
Wave length, selector
sample
Photo detector
Read device
Xenon lamp, H, D discharge incandescent
Filters and/or monochromators
Absorption, intereference
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a
b
c
d
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Start the lamp Start the fluorometer
accessories, Start SpectaAcq Start the
computer, Instrumental Control
Center FL3-11-Generic Layout
System Collect Experiment
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Trial and decide
Trial and decide
Trialand decide
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Emission Acquisition Excitation
Acquisition Synchrous ..
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Caliberation of excitation monochromator
Checking and
For caliberation and its Verification adjust
excitation Spectrometer first
Is the peak at 4670.5
no
yes
Instrument is not within Specification,
Recaliberate excitation monochromator
Instrument is within specifn Go to emission
caliberation
467nm
Slits1.0 Ex 220-600nm Incr0.5 nm Int 0.5 sec
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Checking the Emission acquisition caliberation
Is the peak at 397 0.5
yes
no
Within specification Caliberation is complete
Instrument is not within specification
Continue on to Emission recaliberation
Has the exc. monochromator been Caliberated?
yes
no
Back to Excitation caliberation
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Shah, B. K., Neckers, D. C. Chem. Mater, 2006,
15,603