Jianmin Zhong, Neval Erturk, Stephanie Songer, Tracy Ware

1
The N and C -Termini of Human and Porcine Insulin
B Chains Are Not Involved in Binding to Insulin
Receptor
Jianmin Zhong, Neval Erturk, Stephanie Songer,
Tracy Ware

• I. Background
• 100 identity between human and porcine insulins
• A Structural Overlay of These Insulins with
  Swiss-PDB Viewer Showed Non-Superimposed Termini
  of the Insulins.
• This is Not Surprising Since N and C Termini Are
  Often Unstructured (and Mobile) Regions in
  Protein Structures.

III. Resources Methods a. PubMed/BLAST (NCBI)
to retrieve align insulin receptor protein
sequences b. Protein Data Bank to View
Structures c. ConSurf Server to Align d.
Swiss-PDB Viewer to Align Structures e. Google
(How Does Insulin Bind Its Receptor?) f.
Wikipedia.org g. Beta Cell Consortium for
information on primary binding regions
of insulin (Figure 2)
Figure 1 Overlay of Porcine Human Insulin
Figure 2 Binding Regions of Insulin B Chain
(Green Tan)
II. Hypothesis Unstructured termini of the two
insulins, although 100 sequence conserved
between Pig and Human, is unlikely to be involved
in binding the Insulin Receptor.
Hypothesis Supported!