Advanced Sensors

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Advanced Sensors for Membrane Targets New
Transduction Methods Atul N. Parikh UC
Davis anparikh_at_ucdavis.edu http//parikh.ucdavis.e
du NSF Biophotonics Center NIH Nanomedicine
Center Dept of Energy
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60-80 prescription drugs target membrane
proteins 1/3rd genome codes for membrane
proteins Bacterial toxins, viral entry mediated
by membranes
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Applications
One of the interaction partners is surface
immobilized
Protein-ligand interactions
Drug-target interactions
Membrane protein receptor interactions
DNA hybridization
Cell, viral interactions
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H. Bayley and P. S. Cremer, Nature (Perspective),
2001
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www.unc.edu (Berkowitz Group)
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http//www.chem.unc.edu (Berkowitz)
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PH Dependence of FITC
PH 10
PH 7
PH 4
Dark Green
Bright Green
Green
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Surfactant Removal by Photochemical Calcination
Clark et. al. Chem. Mater., 2000, 12, 3879.
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Pattern Generation by Photochemical Calcination
UV-Light
UV-Light
Metallic Mask
Mesostructured Thin Film ( 1800 Å) On a SiO2/Si
Substrate
SEM Images
Mesostructured
Optical Microscopy
Mesoporous
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pH-dependent properties of fluorescent sol-gel
PH10
PH7
PH4
FITC-F127 Film on Si
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Vesicle Spreading on patterned molecular
monolayers
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Hydrophilic/hydrophobic corrugation
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PH4
PH7
PH10
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PH4
PH7
PH10
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Label-Free Imaging of Membrane Topography and
Protein-Membrane Interactions Photonic Colloidal
Crystals
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Figure 1. ? A Cartoon representations of a
thin-film photonic crystal based detection of
biomolecular interactions. Left panel,
immobilized receptors Right panel,
membrane-mimetic receptors.
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Figure 2. ? An optical image of a 240 nm
self-assembled photonic crystal (A. M. Brozell,
M. A. Muha, A. N. Parikh, submitted, 2005).
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Figure 3. ? (Top) Instantaneous dip in the
spectral intensities measured at 590 nm within
the stop band (intensity at the half-maxima) was
observed upon incubation with Mouse Anti-Goat in
phosphate buffered saline (PBS, 150 nM NaCl).
The colloidal crystals were derivatized with Goat
Anti-Bovine. (Bottom) As a control, similar
crystals were placed in PBS buffer and more
buffer was added at the same rate via syringe
pump. No noticeable change in reflection was
observed.
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Label-Free Imaging of Membrane Topography and
Protein-Membrane Interactions Ellipsometric
Imaging
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Ellipsometric Imaging at the Nanoscale
Label-free Quantitative Real-time Bilayer
topographies Out-of-plane Protein-binding
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Binding of Cholera toxin to Gm1 in Coralled
Membranes
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Label-Free Imaging of Membrane Topography and
Protein-Membrane Interactions Fluidity changes
upon Ligand-receptor interactions
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CTB (1.4 x 10 -7 M)
V. Yamazaki, O. Sirenko, R. Schafer and J. T.
Groves JACS, 2005
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0.5 Gm1/DMPC (bef CTB binding)
0.5 Gm1/DMPC (aft CTB binding)
a.
b.
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Label-free Biosensor Platforms for Membrane
Targets

This work has been supported by funding from the
National Science Foundation. The NSF Center for
Biophotonics Science Technology, an NSF Science
and Technology Center, is managed by the
University of California, Davis under Cooperative
Agreement No. PHY 0120999.