North Carolina State University Oat Maker Development

1
North Carolina State University Oat Maker
Development
Crop Science Department 840 Method Rd, Unit
3 Raleigh, NC 27695

• Peter Maloney
• Jeanette Lyerly
• Paul Murphy
• Gina Brown-Guedira USDA-ARS

2
Winter Hardiness Project

• Goal To develop lines with increased tolerance
  to cold and freezing temperatures and map Winter
  Hardiness Component Traits (WHCT)
• Preliminary Work
• Screened known Oat RFLP and SSR markers in
  Fulghum x Norline RIP phenotyped for WHCT
• Tested Wheat SSR primers for amplification in Oat
• Markers were randomly chosen and had a very low
  success rate
• One oat SSR developed through this screening is
  associated with a QTL for WHCT

3
Winter Hardiness ProjectSSR markers

• Collaborating with Dr. Joe Andersons lab at
  Purdue University to test newly developed SSR
  markers
• Evaluated primers generated by the Anderson lab
  on a panel of 11 different cultivars
• Used the Licor 4300 Analysis System
• Those that were polymorphic for Fulghum x Norline
  RIP were then mapped onto the population
• See poster by Peter Maloney for more
  information

4
Winter Hardiness ProjectSNP discovery

• Sequence PCR fragments
• First SNP developed into CAPS1 marker
• See Linkage Group FN7 for location
• Develop SNP markers from oat ESTs
• Herman et al. (2006) described regions in wheat
  associated with up or down-regulation during cold
  acclimation
• ESTs from these genes were used to develop
  markers that were screened for amplification in
  our panel of oat cultivars
• Through this process 2 SNPs and 1 deletion were
  found with a 4 success rate

( See poster by Peter Maloney)
5
Future Plans

• Develop cDNA libraries using oats that have been
  exposed to sub-zero temperatures
• Develop RIL to study BYDV resistance in oats
• Associate markers with BYDV resistance for marker
  assisted selection
• Develop RIL to study Grain Protein Content
• Identify markers that can be used in marker
  assisted selection for Grain Protein