HPLC for Beginners

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HPLC for Beginners

• Harmit Singh Ph.D.

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Applications

• Pharmaceuticals
• Food
• Nutrition
• All other

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What is HPLC

• High Performance Liquid Chromatography
• High Pressure Liquid Chromatography

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Chromatography
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CHASM

• Charge
• Hydrophobicity
• Affinity
• Solubility and stability
• Molecular weight

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Instrument

• Sample
• Solvent and Pump
• Column
• Detector
• Computer

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Column

• Stationary phase
• Different Packing materials
• Pore size
• Charge
• Hydrophobicity

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Pump and Solvent

• Mobile phase
• Gradient
• Mixed solvents
• MeOH, ACCN, H2O
• pH
• Buffers