FryeEdidin Experiment

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Frye-Edidin Experiment 1970
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Membrane fluidity Frye-Edidin experiment
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Frye-Edidin experiment
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Frye-Edidin experiment
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Lipid bilayer
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Fluid mosaic model of membrane
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Distinct domains in model membranes
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3-D image of lipid microdomains containing
cholera toxin
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Characteristics of lipid microdomains

• Sub-population of cellular membranes
• Insoluble in non-ionic detergents
• Enriched in cholesterol, sphingolipids,
  glycolipids, GPI-anchored proteins and specific
  integral membrane proteins
• More highly ordered membrane areas but still
  dynamic
• Suggested to represent signaling centers, sites
  of endocytosis and pathogen entry

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Typical lipid raft
Cholesterol
Sphingolipid
Phospholipid
Glycosphingolipid
GPI-anchored protein
Lipid Microdomain
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Fluorescence labeling of cholesterol in membrane
microdomains
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Lipid microdomains potential role in polar
secretion
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Western blot analyses of Arabidopsis plasma
membrane fractions
1249 AnnAt2 1250 AnnAt1 E8 pea p35
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