HUNTING DRUGVACCINE TARGETS FOR

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HUNTING DRUG/VACCINE TARGETS FOR Lymphatic
filariasis
Dr. S. L. Hoti, Deputy Director, Molecular
Biology Division, Vector Control Research
Center, Pondicherry - 605 006.
WORLD HEALTH ORGANIZATION Collaborating Centre
for Research Training in Integrated Methods of
Vector Control. Web Site"http//www.pon.nic.in/f
il-free"
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Insilico Comparative Genome Analysis of C.elegans
and B.malayi
Introduction
Human Lymphatic Filariasis (Elephantiasis) Causati
ve organism Human Filarial Nematodes -
Wuchereria bancrofti - Brugia malayi - Brugia
timori In India (socioeconomic burden) it is
responsible for the loss of about 0.63 of the
per capita gross national product.
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Parasites as Agents of Disease Worldwide (WHO TDR
Report1990)
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Infectivity Rate of Different Parasites(1996)
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Lymphatic Filariasis is targeted for elimination
by 2020
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EFFECT OF ANTI FILARIAL DRUG
PARASITE CLEARENCE
MONTHS
Drug fails to clear parasite load completely.
Hence macrofilaricide is required
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Insilico Comparative Genome Analysis of C.elegans
and B.malayi
Comparative Genomics
Brugia malayi
Caenorhabsitis elegans
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Insilico Comparative Genome Analysis of C.elegans
and B.malayi
ESTs (Expressed Sequence Tags)
? Single pass reads from randomly selected cDNA
clones ? 300-500 base pairs ? Sequences for
protein coding regions ? Fragments of genes
Expressing genes as mRNA
Organism
cDNA
ds cDNA
ESTs
cDNA library
Random clones
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Insilico Comparative Genome Analysis of C.elegans
and B.malayi
Methodology
ESTs of C.elegans
http//brie2.cshl.org8081/downloads.html
ftp//ftp.sanger.ac.uk/pub/C.elegans_sequences/EST
S/C.elegans_nematode_ESTs.gz
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Insilico Comparative Genome Analysis of C.elegans
and B.malayi
Methodology
Database Creation
Only B.malayi ESTs
GenBank Format
Microsoft Access
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Insilico Comparative Genome Analysis of C.elegans
and B.malayi
Methodology
For C.elegans ESTs
BioEdit Local BLAST Nucleotide Database
FASTA Format
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Results
Phase
-
III
Target Selection
Comparative Genome Analysis of
Insilico
Comparative Genome Analysis of
Insilico
Comparative Genome Analysis of
Insilico
C.elegans
and
B.malayi
C.elegans
and
B.malayi
C.elegans
and
B.malayi
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Thank you