The mystery of the enolase pocket

1
The mystery of the enolase pocket
E. coli RNase E is a multifunctional protein
complex

• Conclusions
• Two of the four amino acid residues that
  directly interact with the cognate RNase E
  binding site are completely conserved amongst E.
  coli, yeast, and Methanococcus enolases and one
  is similar. Yeast is significantly different at
  the 4th residue position.
• Amino acid alignments alone are insufficient and
  can be misleading in analyzing the functional
  significance of conserved residues in proteins.