Are You Safe in Your Own Lab

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Are You Safe in Your Own Lab?

• Clark Ford
• Jeanne Stewart
• September 3, 2003

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Outline

• Overview of Safety Responsibilities
• Clark Ford
• New Safety Concerns
• Jeanne Stewart
• Jeopardy!

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FSHN Safety Check List

• Review of Chemical Hygiene Plan
• Safety Check-in
• Site-Specific Safety Training
• Standard Operating Procedures
• Approval for Hazardous Lab Operations
• Material Safety Data Sheets
• Yearly Chemical Inventory
• Yearly Lab Safety Survey
• Radiation Safety and BioSafety Checklists

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Safety Check-in

• I have read the Chemical Hygiene Plan
• I have completed a Hazard Inventory
• I have received Site-Specific Safety Training
• I know yearly lab inspections are required
• I understand proper procedures for managing
  and disposal of chemical waste

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Safety Check-in

• I may need to find a buddy for work at night
• I know where MSDSs are in my lab
• I know the number to call if injured
• I know glasses and a lab coat are required
• I understand that the safety of new research
  methods must be discussed

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Safety Check-in

• I understand the Emergency Evacuation Plan for my
  lab
• I know the location of all safety equipment in my
  lab
• First Aid kit
• Fire Extinguisher
• Safety Shower
• Eyewash
• Spill Kits
• Circuit Breakers
• Training Records
• Standard Operating Procedures
• Nearest Phone

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Safety Check-in

• I understand that I must be trained in proper
  procedures to use laboratory equipment
• I understand the lab notebook format required in
  my lab

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Safety Check-in

• I understand that before I leave the lab I must
  complete a check-out involving
• Disposal of all chemical waste and old samples
• Proper storing and labeling of all research
  material left
• Preparation of a complete inventory of research
  material left
• Clean up of my personal workspace
• Updating my lab notebooks
• Inspection

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New Safety Concerns

• Water Aspiration Vacuum cannot be used for
  organic solvent evaporation
• Causes pollution
• Must use mechanical vacuum pump with cold trap

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New Safety Concerns

• Contaminated lab coats must be DECONTAMINATED
  before laundering
• Sterilize in autoclave, or
• Use 10 bleach

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New Safety Concerns

• Alternate Principle Investigator link
• Policy
• Example Checklist

• Department Safety Forms

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Personal Safety for 100

• Kind of Glove to use for chemical work

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Personal Safety for 100

• What are Nitrile Gloves?

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Personal Safety for 200

• Protects your eyes from UV light, dust, solvents,
  liquids under pressure, or flying particles

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Personal Safety for 200

• What are Safety Glasses or Goggles?

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Personal Safety for 300

• What not to use in the lab to
• achieve suction on a pipette
• drain a cup of coffee
• process food or gum

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Personal Safety for 300

• What is your mouth?

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Personal Safety for 400

• Garment that should be worn at all times in the
  lab

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Personal Safety for 400

• What is a Lab Coat?

Also closed-toed shoes
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Personal Safety for 500

• What you should do with your hands before leaving
  the lab

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Personal Safety for 500

• What is Washing ?

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Chemistry for 100

• To avoid splashing when mixing acid and water,
  this liquid is poured into the other

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Chemistry for 100

• What is Acid?

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Chemistry for 200

• Labels on secondary chemical containers should
  contain this information

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Chemistry for 200

• What are
• Name of Chemical
• Solute and solvent
• Safety Warnings
• Initials of Scientist
• Date

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Chemistry for 300

• What you should set bottles of hazardous
  substances in

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Chemistry for 300

• What is a Secondary Containment basin?

Bottle needs cap and waste description tag
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Chemistry for 400

• Substances that should not be vacuum aspirated
  using a water aspirator.

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Chemistry for 400

• What are Organic Solvents?

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Chemistry for 500

• Used to clean up hazardous spills of chemicals
  such as organic solvents, acids, or bases.

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Chemistry for 500

• What is a spill kit?

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Waste for 100

• What you do with gloves before you touch
  doorknobs or telephones?

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Waste for 100

• What is Removing Gloves?

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Waste for 200

• Kinds of liquid waste that you should keep in
  separate labeled bottles

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Waste for 200

• What are organic solvents?

Bottles need cap and waste description tag
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Waste for 300

• What you do with old microbial liquid and plate
  cultures

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Waste for 300

• What is autoclaving biohazards/ microorganisms?

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Waste for 400

• The item that must be filled out and attached to
  a waste chemical bottle

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Waste for 400

• What is a Waste Description Tag?

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Waste for 500

• characteristics used to separate radioactive
  waste into appropriate waste containers

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Waste for 500

• What are
• Isotope?
• Radioactive half life?
• Solvent?

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Procedures for 100

• Where you would get bandaids, gauze pads tape,
  or ointment for a minor wound.

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Procedures for 100

• What is a First Aid Kit?

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Procedures for 200

• Where you (or EHS or EPA) would go to locate your
  current laboratory chemical inventory

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Procedures for 200

• What is your Chemical Hygiene Plan (CHP) Book?

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Procedures for 300

• What you should be able to find in your lab with
  your eyes closed

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Procedures for 300

• What is the location of the eyewash station?
• (and all other safety equipment)

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Procedures for 400

• Where you (or EHS or the Fire Department) can
  quickly find phone numbers of people responsible
  for safety in a lab.

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Procedures for 400

• What is the Emergency Contact Card on the lab
  door?

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Procedures for 500

• In an emergency, the number you would call for
  fire or injury

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Procedures for 500

• What is 911?

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Explosions for 100

• Place where organic solvents are stored at room
  temperature

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Explosions for 100

• What is a Flammable Storage Cabinet?

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Explosions for 200

• This needs to be consigned to waste after 90 days
  due to peroxide formation

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Explosions for 200

• What is Ethyl Ether?

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Explosions for 300

• Laboratory equipment that must be secured to a
  counter so that it wont fall and become a
  projectile

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Explosions for 300

• What is a gas tank?

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Explosions for 400

• Where you must store refrigerated or frozen
  samples containing organic solvents or peroxides

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Explosions for 400

• What is a Flammable Materials Storage (Explosion
  Proof) Refrigerator/Freezer?

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Freezer Explosion in HNSB

• A freezer exploded in HNSB in 2001.
• The door was blown off the freezer
• The shock wave damaged the building structure
• A fire started on the top shelf of the freezer
• Sprinklers came on and stayed on for 30-60
  minutes

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Explosions for 500

• Your first reaction to a spill of flammable
  materials near a heat or ignition source

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Explosions for 500

• What is turning off the heat or ignition source?

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Fire and Explosion in 1985

• A hot plate stirrer in 223C MacKay had an
  electrical short
• Fire burned through a 2 inch wood lab bench
• Ether vapors from samples stored in a
  non-explosion proof refrigerator exploded
• Fire spread to entire lab