A circular piece of DNA known as a bacterial plasmid or vector may be used to propagate recombinant

1
A circular piece of DNA known as a bacterial
plasmid or vector may be used to propagate
recombinant DNA sequences. http//www.sci.sdsu.ed
u/smaloy/MicrobialGenetics/topics/chroms-genes-pr
ots/supercoiled-plasmid.gif
2
A circular piece of DNA known as a bacterial
plasmid or vector may be used to propagate
recombinant DNA sequences. www.cofc.edu/petersj/
plasmid.jpg
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The human genome project has been completed and
now we know the sequence of all of the genes in a
human cell. http//libproxy.mit.edu8791/nature/j
ournal/v421/n6921/fig_tab/nature01403_F6.html
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The genome center located in the Whitehead
Institute played a large role in the public human
genome project.
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Eric Lander runs the genome center here on campus.
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Salvador Luria
Salvador Luria discovered restriction enzymes
proteins capable of cutting DNA at distinct
sequences. http//nobelprize.org/medicine/laurea
tes/1969/luria-bio.html
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Salvador Lurias experiment with bacteriophage
identified the existence of restriction
enzymes. micro.magnet.fsu.edu/ cells/virus.html
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Salvador Lurias experiment with bacteriophage
identified the existence of restriction
enzymes. http//www.agen.ufl.edu/chyn/age2062/
lect/lect_06/lect_06.htm
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When plasmids containing a drug resistance marker
are transformed into bacteria, the bacteria
divide several times forming colonies able to
grow on the drug.
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SOME RESTRICTION ENZYMES (NEBL)
AsiS I GCGATCGC Ava I
CYCGRG Avr II CCTAGG Bae I
AC(N4)GTAPyC BamH I GGATCC Ban I
GGYRCC Ban II GRGCYC Bbs I
GAAGAC Bbv I GCAGC BbvC I
CCTCAGC Bcc I CCATC BceA I
ACGGC Bcg I CGANNNNNNTGC BciV I
GTATCC Bcl I TGATCA Bfa I
CTAG BfrB I ATGCAT BfuA I
ACCTGC BfuC I GATC
Aat II GACGTC Acc65 I GGTACC Acc
I GTMKAC Aci I CCGC Acl I
AACGTT Afe I AGCGCT Afl II
CTTAAG Afl III CRYGT Age I
ACCGGT Ahd I GACNNNNNGTC Ale I
CACNNNNGTG Alu I AGCT Alw I
GGATC AlwN I CAGNNNCTG Apa I
GGGCCC ApaL I GTGCAC Apo I
RAATTY Asc I GGCGCGCC Ase I
ATTAAT
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This is a commonly used cloning vector. It
contains an origin of replication, an ampicillin
drug resistance marker, and a restriction
site. http//photoscience.la.asu.edu/photosyn/cou
rses/BIO_343/lab/plasmid.gif