PowerPointPrsentation

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http//barleygenome.org/
progress towards efficient gene isolation and
genomic sequencing in barley
Mission statement Barley (Hordeum vulgare L.) was
one of the first domesticated cereal grains,
originating in the Fertile Crescent over 10,000
years ago. Barley ranks fifth in worldwide crop
production and is widely cultivated in all
temperate regions from the Arctic Circle to the
tropics. In addition to its geographic
adaptability, barley is particularly noted for
its tolerance to cold, drought, alkali, and
salinity. The barley genome - with 5.3 billion
letters of genetic code - is one of the largest
in cereal crops measuring about twice the size of
the human genome. Barley is a true diploid, thus,
it is a natural archetype for genetics and
genomics for the Triticeae tribe, including
polyploid wheat, and rye. Highly collaborative
international efforts have produced a substantial
body of genetic and genomic resources in the past
several years. The objective of IBSC is to
physically map and sequence the barley gene space
including the 5 and 3 regulatory regions of
genes. The central goal is to develop an ordered
physical map linked to the genetic map in order
to accelerate gene isolation and crop improvement
and to provide the basis for the longer-term goal
of sequencing the barley genome.
Members Australian Center for Plant Functional
Genomics (ACPFG) Leibniz Institute of Plant
Genetics and Crop Plant Research (IPK) Peter
Langridge Andreas Graner (chair), Nils Stein
(vice-chair) MTT Agrifood / University of
Helsinki Scottish Crop Research Institute
(SCRI) Alan Schulman Robbie Waugh Okayama
University University of California,
Riverside Kazuhiro Sato Timothy J
Close National Institute of Agrobiological
Sciences (NIAS) USDA-ARS / Iowa State
University Takashi Matsumoto Roger Wise
Developing a physical map by High Information
Content Fingerprinting (HICF) target 15x
haploid genome coverage
Anchoring the physical map to the high-density
and high-resolution genetic map of barley
mapping of up to 10 - 15,000 barley genes, BAC
end sequencing and mapping, colinearity to model
genomes
Research Agenda
Sample sequencing the barley genome
Gene-enriched library sequencing (GSS), contig-
and sample-BAC sequencing
Contig-based and shotgun whole genome sequencing
Physical map construction HICF of 83000
gene-containing genes, collaborative project
between UCR (TJ Close) and UC Davis (MC Luo) -
expiry end 2007, funding NSF Plant Genome
Research Program 10x genome coverage HICF
ongoing as collaborative project between IPK,
ACPTG and TU Munich - ongoing (2007-2009),
funding Leibniz Association, ACPFG 200,000 BAC
clones HICF - ongoing 2007 2010, funding
BMBF anchoring physical map for chromosomes 1H
and 3H - approval pending, expected starting
date 2008, funding EU-FP7
Ongoing Activities
Genetic mapping 3000 ESTs mapped via SNP,
Okayama University (K Sato) - completed 1000 EST
consensus map, IPK (A Graner) - completed 2500
EST mapped via SNP ILLUMINA Golden Gate Assay (TJ
Close, R Waugh, N Stein) - Completed. Funded by
NSF Plant Genome Research Program and BarleyCAP
(TJC), AGOUEB (RW), IPK core funding and
BMBF/GABI (NS). 4000 unigenes via SFP and eQTL
analysis (R Waugh, R Wise) - completed
Sequencing 25,000 full length cDNAs, NIAS (T
Matsumoto, K Sato) - funding available,
ongoing 500 chromosome 3H BACs of cv. Haruna Nijo
(K Sato) - funding available, ongoing 250,000 BAC
clones end sequencing (SCRI, IPK, Udine
University) - funding available ERA-PG 3000 BACs
low-pass shotgun sequencing - funding
available, BMBF