Jay Nadeau1, Victor White1, Joshua Maurer2, Dennis Dougherty2, James Beddow3, Ian Peterson3 1 Jet Pr

1
Jay Nadeau1, Victor White1, Joshua Maurer2,
Dennis Dougherty2, James Beddow3, Ian Peterson31
Jet Propulsion Laboratory, California Institute
of Technology2 Department of Chemistry,
California Institute of Technology3 Center for
Molecular and Biomolecular Electronics, Coventry
University, UK
Biomolecular Systems Research Program
Toward a fully enclosed ion channel sensor
Questions is the behavior lipid dependent? Is
there more than one isoform? Are there better
methods of purification?
Ion channel recordings with agarose
Left, alamethicin in agarose at different time
scales (top, 60 s per sweep middle, 10 s per
sweep, bottom, 2 s per sweep). Right,
all-points histogram of sweeps showing different
conductance states.
Micrographs of SiN-SU8 orifices

• Conclusions and Future Work
• Fully enclosed device will consist of 2 chips
• Stability and lifetime need to be tested
• Chimeras will be tested for gating and function
• GlyR purification and perhaps mutation look
  promising

Capacitance measurements/bilayer formation
With SU(8) ? .06 ms A 1.2 nA C 7.2
pF SU(8) Lipid ? .6 ms A 11 nA C 67
pF ?????? ? .096 ms A 90 nA C 850 pF