BIOREACTOR OPERATION PARAMETERS AS TOOLS FOR METABOLIC REGULATIONS IN PRODUCTION

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CATALYSIS through METABOLIC ENGINEERINGBIO-CHE
MICAL ENGINEERING for INDUSTRIAL
BIO-TECHNOLOGY DEVELOPMENT
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• Research in
• INDUSTRIAL BIOTECHNOLOGY METABOLIC
  ENGINEERING
• is based on the basic principles of
• chemical engineering,
• e.g. reaction engineering, momemtum-, heat-,
  and mass- transfer
• in relation with the basic sciences,
• e.g. mathematics, physics, chemistry (physical
  chemistry and biochemistry), biology (industrial
  microbiology and genetic engineering).

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Q-1 What can be produced by
biotechnological processes? A-1
Biomolecules. Q-2 Which industries produce
biomolecules? A-2 Bio-chemical process
industries.

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Q-3 What are the bioprocess
industries (sectors) producing biomolecules?
A-3 Biomolecules are produced by1.
Pharmaceutical biotechnology industrye.g.
antibiotics, vitamins, therapeutic proteins2.
Enzyme production industrye.g. proteases,
glucose isomerase, benzaldehyde lyase3. Basic
biochemicals industrye.g. amino acids, organic
acids, ethanol4.Classical biotechnology
industrye.g. Beer, wine, yeast.

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• Q-4 How  biomolecules are produced? A-4
  By using microorganisms (or plant cell lines,
  and animal cell lines) as
• micro-bioreactors within bioreactor systems.

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Q-5 How  biomolecules are produced?A-5 In the
cell...
medium composition pH T DO
cell micro-bioreactor
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... through intracellular biochemical reaction
network
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• Q-6 Do chemical engineering students need any
  other background?
• A-6 Graduate program students, e.g, MSc, have to
  take seven courses, two of which can be selected
  from under-graduate program courses.
• Under-graduate courses related with
  molecular genetics, microbiology and
  biochemistry are appropriate for those planning
  to work in bio-chemical engineering reserach
  fields.

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• Q-7 Which concepts of chemical engineering
  education,
• find applications of theory?
• A-7 Bio-chemical engineering is based on
  basic principles of chemical engineering. You
  will find the applications for
• Reaction engineering (e.g. calculation of
  intracellular reaction rates, and structured
  kinetic models for production in the bioreactor)
  
• Mass and heat transfer (e.g. mass and energy
  balances)
• Transport phenomena (e.g. mass transfer
  coefficients)
• Separation processes (e.g. high technology
  separation processes, affinity chromatagrapyh,
  and ultra-filtration)
• Process control (e.g. PID control of
  measurements in the bioreactor)

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• Q-8 Can students from basic sciences
  (genetic engineering, biology, chemistry and
  mathematics) achieve graduate work in my research
  group?
• A-8 YES. Through multi-disciplinary graduate
  programme e.g., Graduate Programs of
• Biotechnology, and
• Biomedical Engineering (Molecular Engineering
  Track)

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• Q-9 Can graduates from other engineering
  disciplines achieve graduate work in my research
  group?
• A-9 YES. Through Chemical Engineering and
  multi-disciplinary graduate programs, e.g.,
  Graduate Programs of
• Biotechnology, and
• Biomedical Engineering (Molecular Engineering
  Track)