A DNA sequence of Chlamydia, placed to music

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A DNA sequence of Chlamydia, placed to music

• Ryan Roopnarine
• Cody
• Sam

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The sequence we selected

• Chlamydia
• Why Chlamydia?
• Genbank does not have a spellcheck enabled
  search, and we were unable to spell either
  Gonorrhea off of the top of our heads

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Genbank entry for Chlamydia

• Indicates no distinguishing features for the
  sequence of chlamydia that we selected
  (introns/exons, et cetera), as indicated by the
  /unknown in the features entry of our sequence.

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(No Transcript)
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• An example of a sequence with actual features
  to it..

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This creates a problem for us.

• We dont really have any portions of significance
  to include
• Very little of the sequence itself is melodic, we
  would only be using 25, at most, of it.

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How the tool (we selected) works.

• The online tool that we used (Musicalgorithms)
  takes the interpreted values of each chunk of
  the sequence, and assigns it a numeric value
  between 0 and 3.
• The numeric value of the song is then relatively
  scaled into numbers between 1 and 88, which is
  the number of keys on a piano. If A0, G might
  equal 3 initially, but 88 finally.

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Arrangement of sequences

• Essentially, we made the sequence
• gcgagttacg aagacaaaac ctcttcgttg accgatgtac
  cttgtagaa agtgcataaa
• cttctgagga taagttataa taatcctctt ttctgtctga
  cggttcttaa gctgggagaa
• agaaatggta gcttgttgga aacaaatctg actaatctcc
  aagcttaaga
• cttcagagga gcgtttacct ccttggagca ttgtctgggc
  gatcaaccaa tcccgggc
• Look like
• aacaaatctg aagacaaaac aagcttaaga accgatgtac
  agaaatggta actaatctcc
• agtgcataaa taatcctctt taagttataa tcccgggc
  ttgtctgggc ttctgtctga
• tcttgtagaa cttcagagga cttctgagga ctcttcgttg
  ccttggagca cggttcttaa
• gatcaaccaa gcttgttgga gctgggagaa gcgagttacg
  gcgtttacct
• Where a0, t1, c2, and g3

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Our desired result

• Ultimately, we were trying to create an effect in
  which each 10 letter base combination was a kind
  of chromatic scale (a scale where all of the
  notes in between two intervals are played)
• Eg. B-scale (B-flat, B, B)
• Each successive scale (10 letter group) would
  get higher in register.

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• We felt that if our sequence could not be of
  aesthetic value as a piece of music, that it
  could be of statistical value as such, as one can
  somewhat tell how the pitch goes up as the song
  progresses.
• By making our sequence into a scale, we were able
  to create a song in which all of the genetic
  instructions are preserved, as opposed to cutting
  segments outor repeating them.

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Origin of our sequence

• http//www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db
  nuccoreid62911037feature_62911037
• Our sequence came about from experiments in which
  a new method for detecting Chlamydia infections
  in pregnant women was evaluated.