Recombinant DNA Sea slugs

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Recombinant DNA Sea slugs
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What would you do?

• One day your brother or sister or friend is
  peeing a lot.
• Theyre also really thirsty.
• What would you say?
• Okay so then theyre still peeing and drinking a
  lot of water all week and then they complain of
  blurred vision and theyre breath starts to smell
  like acetone (nail polish remover).
• What do you do?
• The doctor tells you they have diabetes.

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Diabetes

• If you have diabetes your body doesnt make
  insulin.
• Insulin tells cells to absorb sugar and burn it
  for energy.
• Without sugar to burn your body uses fat while
  the sugar lies around in the blood causing
  problems.

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Where does the insulin come from?

• We used to get it from animals but animal insulin
  isnt exactly human insulin and side effects like
  rashes would happen.
• In 1978 a company started using E. Coli Bacteria
  that has recombinant DNA in it that makes it make
  insulin for us like a little insulin factory.

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Wheres the money?

• One major supplier of insulin isEli Lilly
• Fortune 500 corporation,
• revenues of 14.6 billion in 2005,
• 148th largest company in the U. S.
• prozac, cialis, and were the first to distribute
  methadone as an alternative for heroin users
  trying to quit.

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Who else makes Insulin

• Other major distributor is Novo Nordisk
• H.Q. in Denmark,
• production facilities in six countrieswith
  affiliates or offices in 68 countries.
• Employs 22500 people globally (as of 2005) and
  had a turnover of 33.8 billion Danish kroner in
  2005 (EUR 4.5 billion). They made 920 mil in
  2004.

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Recombinant DNA

• an artificial DNA sequence resulting from the
  combining of two other DNA sequences in a
  plasmid.
• Plasmids are little chunks of DNA that copy
  themselves.
• Recombinant proteins are proteins that are
  produced by different genetically modified
  organisms following insertion of the relevant DNA
  into their genome.
• As this recombines the DNA of two different
  organisms, the word recombinant is used to refer
  to this process.

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What are sea slugs?

• Molluscs without the shell
• Interesting defense ability to eject their
  sticky intestines out onto predators which
  grosses them out.
• Also have stigners
• Some of the most colorful creatures on earth

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• Recently the people who make insulin were trying
  to use recombinant DNA in other animals and they
  found that these Krotecus Snails could remove
  human waste from water in a matter of weeks.

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• If you work at Eli Lilly as a scientist what will
  you have to do before you can go to someone and
  convince them to spend money on this?
• Use the scientific method.
• Observe, form a hypothesis with a prediction as
  to how your experiment will credit or discredit
  your hypothesis, experiment, collect data,
  analyze data, accept or reject your hypothesis
  and let people know about it.

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• How do we know theyre alive?
• Students will make a list of the properties that
  all living things share. Students will share the
  list and the instructor will write what they
  suggest on the board.

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• Properties of living things
• Organization Cells,
• Respond to stimuli in environment,
• Homeostasis,
• Metabolism,
• Growth Development,
• Reproduce.
• Change over time or evolve.

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How do you know you can trust this claim?

• Critical thinking
• Use baloney detection