Translation, Translocation of Viral Membrane Glycoproteins

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Translation, Translocation of Viral Membrane
Glycoproteins

• Outline of Lecture
• Description of Steps in the Process
• How to Search the Primary Literature
• Protein Dynamics
• Membrane Traffic
• Fusion and Fission Machinery

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Translation, Translocation of Viral Membrane
Glycoproteins

• Basic Steps in Viral Glycoprotein Synthesis
• Signal sequence targeting to ER, complete
  translocation
• Folding, reduction and glycosylation of protein
• Concentration at exit sites
• Movement to Golgi
• Processing in Golgi
• Sorting and transport to appropriate target
• Assembly and budding of final virion

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Protein and Membrane Dynamics
Protein Concentrations and Membranous Organelle
Sizes Because proteins and membranes turn over
very rapidly (1-10 hours for some proteins and
for membrane organelles), their content in the
cell is determined by similar equations (at
steady state, the rate of gain equals the rate of
loss)
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Protein Levels at Steady State
At steady state dProtein/dt 0 then (rs
rd)dt/No dV/V where rs translation rate
(1 error frequency) rd degradation rate, No
number of molecules/cell and V is the cell
volume rd Protein kd where kd is the
first order degradation rate constant
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How to Approach References
We will start now to look at original papers in
this area and that will mean searching the
literature through PubMed either directly
(http//www.ncbi.nlm.nih.gov/entrez/query.fcgi?db
PubMed) or indirectly through EndNote (Ref.
Program for Word). Presley, J.F., N.B. Cole,
T.A. Schroer, K. Hirschberg, K.J. Zaal, and J.
Lippincott-Schwartz. 1997. ER-to-Golgi transport
visualized in living cells. Nature. 38981-5.
http//www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd
RetrievedbPubMeddoptCitationlist_uids9288971
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