advancing the state of the art in microscopy

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Imaging Protein Crystals and Managing
the Associated Data
advancing the state of the art in microscopy
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• What Type of Microscope to Use?

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• What Type of Microscope to Use?
• Sample
• Liquid Drop w/ Solid and/or Semi Solid

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• What Type of Microscope to Use?
• Sample
• Liquid Drop w/ Solid and/or Semi Solid
• Sample Location
• MicroBatch, Sitting Drop or Hanging Drop

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Light Source
Sitting Drop Or Microbatch Drop
Microscope
Inverted Configuration
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Microscope
Hanging Drop
Light Source
Upright Configuration
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Sitting Drop Imaging Comparison
Upright
Inverted
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• What Type of Microscope to Use?
• Sample
• Liquid Drop w/ Solid and/or Semi Solid
• Sample Location
• MicroBatch, Sitting Drop or Hanging Drop
• Illumination
• Koehler

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• Properties of a Drop
• - Lens Effect

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• Properties of a Drop
• - Lens Effect
• - Side Wall/Refraction Ring

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Less than 90º
Contact Angle of a Drop
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Hemispherical
Contact Angle of a Drop
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Greater than 90º
Contact Angle of a Drop
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High Contrast More Detail
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Crystals at the Drop Edge - use medium contrast
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Very dark side-wall effect - use low contrast
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Focusing on condensation
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Focusing on condensation
Focusing on Crystals
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High Contrast
Low Contrast
Focusing on Crystals
Focusing on condensation
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Data Management
L.I.M.S.
Laboratory Information Management System
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Barcodes
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Crystallization Data
Experiment Details
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Crystallization Data
Experiment Details Image Management Scoring
Images
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Crystallization Data
Experiment Details Image Management Scoring
Images Reviewing Results and Trends
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Crystallization Data
Experiment Details Image Management Scoring
Images Reviewing Results and Trends Remote
Viewing
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Crystallization Data
Experiment Details Image Management X-Ray Data
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Crystallization Data
Experiment Details Image Management X-Ray
Data Printing Results
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