Cell by Date

1
Cell by Date

• Bio-Indicator for Meat Spoilage

2
Introduction
3
Introduction
4
Introduction
5
Problem with Food Industry
Specification
Design
Modelling
Implementation
Testing/Validation

• PROBLEM Printed sell-by date is based on
  predictive challenge testing
• Not reflective of product exposure to environment
• Printed Sell By Date is unreliable
• SOLUTION Monitor level of exposure and report it
  
• Tell Consumer when meat is not fit for
  consumption

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Motivations
Specification
Design
Modelling
Implementation
Testing/Validation

• Fresh meat is a highly perishable food
• Survey studies show temperature conditions gt10C
  are usual during transportation, retail storage,
  and consumer handling.
• 1/3 of Adults use spoilt food
• Colouring and packaging methods are deceptive
• Win-win situation for producers-consumers
• Recycling of old projects pH, temperature, BFP

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Specification
Specification
Design
Modelling
Implementation
Testing/Validation

• Report When food is off
• Report cumulative Temperature exposure of food
• Temp is largest factor in spoiling
• Easiest way to report if food is bad
• Easy To Use
• Visual Reporter Wow ! factor
• Improve Accuracy of other attempts
• SSM, Intelligent Cooling, Antimicrobial
• Only Threshold devices
• System must be stable for length of shelf-life
• No contact with food
• Need to obey HSA standards

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Considerations/Problems
Specification
Design
Modelling
Implementation
Testing/Validation

• Report Cumulative Temperature exposure of food
• Need memory to do this / Integrator fct
• Visual Reporter
• Needs to have long degradation time
• Improve Accuracy
• Limited Crosstalk with chassis
• Easily tuneable threshold
• Stable for length of Shelf Life
• HSA standards
• Project must not contaminate food

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Solutions
Specification
Design
Modelling
Implementation
Testing/Validation

• Integrator
• Improved Accuracy
• Stability
• HSA standards
• Long Degradation Time
• Firefly FP

Cell Free System
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Solutions
Specification
Design
Modelling
Implementation
Testing/Validation

• Cell-free system
• Widely-used in vitro tool used to study
  biological reactions
• Less of the complex interactions cf. whole cell.
• Provides molecular machinery that can be used in
  reactions with the absence of many cellular
  components.

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Solutions
Specification
Design
Modelling
Implementation
Testing/Validation

• Integrator
• Improved Accuracy
• Stability
• HSA standards
• CFS increases stability due to lack of degrading
  enzymes
• CFS stable for up to 4 days
• CFS agrees with HSA standards

Cell Free System
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Design
Specification
Design
Modelling
Implementation
Testing/Validation
Temp. Increase
Cell-free extract medium
O2
Luciferin
LIGHT!!!
Photinus luciferin O2 ATP ltgt oxidized
Photinus luciferin CO2 AMP diphosphate
light
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Modelling
Specification
Design
Modelling
Implementation
Testing/Validation

• Concept of predictive microbiology
• Involves knowledge of microbial growth responses
  to environmental factors
• Expressed in quantitative terms using
  mathematical equations
• Data used to interpret the effect of varying
  conditions on microbial growth.
• Integrative Temp function of product
• Leads to intelligent product management systems
• Optimization of food quality and safety at the
  time of consumption

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Implementation
Specification
Design
Modelling
Implementation
Testing/Validation
Simplest System Phase 1
Bio indicator
Colour scheme
BAD
Good
OK
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Implementation
Specification
Design
Modelling
Implementation
Testing/Validation

• Simplest System Phase 2
• Discrete Change In addition Phase 1

Good
Bad
Eg. Discrete Change At 25oC for more than 2 hours
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Testing
Specification
Design
Modelling
Implementation
Testing/Validation

• Focus on proof of concept
• Period of 24 hours as opposed to 4 days
• Regulated luciferase synthesis that corresponds
  to predicted outcome
• Light intensity as a measurement (compare with
  colour scheme strip)

17
Time line

• Week 3-4 Modelling GeneART
• Week 4-6 Experiment implementation
• Week 7-8 Testing / Validation
• Week 9-10 Polish the project documentation

18
Conclusion

• Cell by date
• Mimics printed sell by date with additional
  temperature exposure tracking
• More accurate than conventional method
• Will improve consumer health
• Focuses on use of experimentation proof of
  concept

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End

• Questions?