A Biomimetic O2 Carrier: Cosalen

1
A Biomimetic O2 Carrier Co(salen) Synthesis And
Kinetics
Objectives --Synthesize Co(salen)
--Measure kinetics of O2 uptake
--Determine stoichiometry of oxygen complex
2
Oxygen Binding Co Environment

• As Solid

• Solvated

3
Kinetics of Oxygen Uptake

--Bring timer to lab!
--Grind about 30 seconds bring acetone-saturated
towel.
--At least 30 mg combine or divide samples,
work as partners.
--Oxygen flush at least seven minutes.
--Oxygen-saturated DMSO use less for lower
amounts of complex.
4
Kinetics of Oxygen Uptake
--Syringe addition of DMSO not necessary
desirable. Doesnt need strict oxygen
atmosphere.
--Take readings immediately DMSO added, and when
returned to bench.
--Try to attach apparatus at height that reaction
can be stirred. If not, raise flask.
5
Kinetics of Oxygen Uptake
--Share complex if active.
--Do not let water enter flask through sidearm.
--Expect water volume drop of 3 - 20 mL.
--Stop at twenty minutes.
6
Possible Reasons for Inactivity
--Could slowly take up oxygen in solid state.
--Bound ethanol or water remains.
--Not fully dissolved in DMSO.
--DMSO does not completely convert to active form.
7
Kinetics of Oxygen Uptake
--Extrapolate to best end of water uptake.
--Volume water uptake volume oxygen uptake.
--PV nRT moles of oxygen uptake.
--Unreasonable ratios 1) accuracy of
measurement 2) inactive complex
8
Summary
--Bioinorganic chemistry study of a biomimetic
compound that reversibly binds oxygen
--Kinetics measurements to determine solution
structure