Optimizing the Film Matrix for a Fluorescence Based Oxygen Sensor Using PtII Octaethylporphine

1
Optimizing the Film Matrix for a Fluorescence
Based Oxygen Sensor Using Pt(II) Octaethylporphine

• Gregory Smith
• Department of Biomedical Engineering, University
  of Texas, Austin, Texas 78712

2
Background

• Fluorescent dyes used (RITC, Rhodamine, and PtOEP
• Oxygen quenching
• The sensor theory
• The ideal film

3
Testing Apparatus
Spectrometer
Light Source (460nm LED)
Fiber optic cables
Flow in
Flow out
Sample to be tested
4
Flow Chamber
5
TEOS Solution Tests
6
TEOS Film Tests
7
PEG-DA Hydrogel Tests
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E-6000 Adhesive Slides
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E-6000 Adhesive Tests
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E-6000 Adhesive Tests
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PDMS Test
12
Conclusions

• RITC is better than Rhodamine B
• The trade off between signal strength and film
  thickness
• The E-6000 Slides
• The Ideal film is obtainable

13
Future Work
14
Acknowledgements

• Louisiana Tech University
• Departments of Biomedical Engineering and
  Chemistry
• Dr. Sven Eklund
• Dr. Steven Jones
• Damien Davis
• The NSF summer REU program

15
References

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