Fig' 16'34

1
Fig. 16.34
2
Fig. 16.35
3
Nitrate transport

• NRT2 inducible (low N) high affinity
  transporter
• NRT1 family nitrate transporters of high and
  low affinity

4
Fig. 16.36
5
Two H might be transporter along with nitrate
Fig. 16.37
6
Nitrate reductase

• cytosolic
• Found in the root or shoot (or both)
• NO3- NADPH H
  NO2- NADP H2O

7
Nitrate reductase

• Activity appears to be limiting in some cases
  (more often than nitrite reductase for example)
• Highly regulated

8
Nitrate reductase
Fig. 16.38
9
Fig. 16.39
Molybdenum cofactor of NR
10
FAD domain of NR
Fig. 16.40
11
The expression of NR is regulated
Fig. 16.41
Nitrate added at time zero
12
Nitrite reductase

• In plastids
• Uses reduced ferredoxin as the electron donor
• Reduced ferredoxin comes from the electron
  transport chain in leaves or is reduced by NADPH
  in root tissue

13
Fig. 16.42
14
The activity of nitrite reductase is not thought
to be limiting
Fig. 16.43
15
Fig. 16.
16
Fig. 8.04
17
Fig. 8.05
18
Net reaction of GS/GOGAT

• Glutamine synthase
• glutamate NH3 ATP glutamine ADP Pi
• GOGAT
• Glutamine alpha ketogluarate
• 2 glutamate

19
Fig. 8.07
20
Fig. 8.06
21
Fig. 8.08
22
Fig. 8.09
23
Fig. 8.10
24
Fig. 8.11
25
Box 16.1
26
Box 16.1b
27
(No Transcript)
28
(No Transcript)
29
Nitrogenase reaction
N2 16 ATP 8e- 8H ------- 2NH3 H2 16ADP
16 Pi
Reaction 16.1
30
Fig. 16.04
31
Fig. 16.06
32
Fig. 16.07
33
Fig. 16.09
34
Fig. 16.10
35
Fig. 16.11
36
Box 8.2
37
In Arabidopsis there are five different aspartate
aminotransferases, including plastid, cytosolic,
peroxisomal and mitochondrial forms.
Fig. 16.16
38
Fig. 16.18
39
Box 8.2
40
Fig. 16.09
EPSP synthase
41
Fig. 8.24
42
Fig. 8.25
43
Fig. 16.09
44
Fig. 8.27
45
Fig. 8.28
46
Fig. 8.29
47
Fig. 8.30
48
Fig. 8.32
49
Fig. 8.33
50
Fig. 8.33
51
Box 8.5
52
Fig. 8.33
53
Box 8.7
54
Box 8.7
55
Fig. 8.42
56
Box 8.2
57
Fig 8.2
58
Fig. 8.44
59
Fig. 8.49
60
Fig. 8.50
61
Fig. 8.51
62
Box 8.2
63
Fig. 8.52
64
Fig. 8.53
65
Fig. 8.54
66
Fig. 8.54