Introductory Genetics 3A Principle of Linkage Mapping

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Introductory Genetics 3APrinciple of Linkage
Mapping

• Greg Gibson
• Department of Genetics
• North Carolina State University

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Types of Map used in Genetics
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Linkage
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Constructing a Linkage Map
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Linkage Exercise
A cross involving 968 F2 progeny of a cross
between a normal tree and one that produces
small, sour, red, thick-skinned oranges results
in the following combination of phenotypes.
Generate a genetic map involving the four
recessive markers.
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Exercise Answer
Completely linked loci would be expected to
produce about 242 trees of each double-mutant
class, whereas unlinked loci should only have ¼ x
¼ x 968 60 double mutants.
thickskin reddish sour
petite
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Constructing a physical map
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Restriction mapping
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Radiation Hybrids
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Synteny (cat-human)
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Synteny (mouse-human)
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Synteny (rice-cress)
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Pedigree mapping