Enzymes

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Chapter 8 Enzymes!!
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Properties of Enzymes
Proteins
Catalysts
Exquisitely specific--stereospecificity!!
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(OMPOrotidine monophosphate involved in UMP
synthesis)
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Proteases e.g. ?
Trypsin
Chymotrypsin
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Stryer Fig. 8.1
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Stryer Fig. 8.2
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?Go -2.303RT log Keq
?G ?Go 2.303RT log Keq
?Glt0
spontaneous
?G0
At equilibrium
?Ggt0
Reverse reaction is spontaneous.
Adjusting the concentrations of reactants and
products can cause them to happen.
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Stryer Fig. 8.3
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Stryer Fig. 8.4
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Stryer Fig. 8.5 Cytochrome P-450
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Stryer Fig. 8.6 Change in absorption of pyridoxal
phosphate.
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Stryer Fig. 8.7 Active site residues of lysozyme
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Stryer Fig. 8.8 H-bonds between E and S for
ribonuclease.
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Stryer Fig. 8.9 Lock and key.
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Stryer Fig. 8.10 Induced fit.
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Stryer Fig. 8.11
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Stryer Fig. 8.12 Determining initial velocity
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Stryer Fig. 8.13
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Enzyme Kinetics
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K2
K1
E S ?ES?EP
K-1
(K-1 is negligible until products start to build
up)
Vo K2ES
K1ES k-1ES k2ES (k1 k2)ES
ES/ES (k-1 k2)/ k1 KM
E ET -ES
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