Another important feature of the genetic code:

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Another important feature of the genetic
code It is non-overlapping.
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Major features of transcription in
eukaryotes 1. Functionally specialized RNA
polymerases a. RNA Pol I. Large ribosomal
RNAs b. RNA Pol II. Protein encoding
genes c. RNA Pol III. Transfer RNAs,
small ribosomal RNAs,
several other small RNA types. 2. Single
gene transcripts (not polycistronic). 3.
Transcript processing, including a. Intron
splicing b. Capping c. PolyA tails

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There are 64 - 3 61 codons. This might imply
that there are 61 anticodons, and therefore 61
different tRNA molecules. Is this really the
case? There are actually fewer than 61 tRNA
molecules. A single anticodon (tRNA
molecule) can frequently pair with more than
one codon---partially explaining the degeneracy
of the code. The rules of WC base pairing are
relaxed for the 5 base of the anticodon
(corresponding to the 3 base of the codon).
This base is less constrained than the others, it
can form a greater variety of hydrogen bonds.
In Cricks terms, it can wobble.
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Wobble rules for codon-anticodon pairing in E.
coli Anticodon 5 prime base
Codon 3 prime base A
U
C
G U
A or G G
C or U
I
A or C or U
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