Research Tissue

1
Research Tissue

• Kate Truncale, MS
• Senior Scientist

2
Overview

• About MTF
• Why Research Consent is Important
• Research Tissue Avenues
• Examples of Various Research Projects Utilizing
  Research Tissue

3
MTF Mission Statement

• MTF is a non-profit service organization
  dedicated to providing quality tissue through a
  commitment to excellence in education, research,
  recovery and care for recipients, donors their
  families.

4
MTF Facts

• MTF was founded by leading academic orthopedic
  surgeons who identified an increasing need for
  high quality allograft tissue.
• Since 1987, MTF has recovered over 60,000 donors
  and have distributed over 3 million grafts.
• In 2007, MTF recovered over 6,300 donors
  resulting in more than 390,000 grafts to
  surgeons, hospitals, dentists and clinicians.

5
Why We Need Research Consent

• Honor maximize the gift
• If donor is non-transplantable and does not have
  research consent it cant be used!
• Improve current tissue forms processes
• Develop new tissue forms processes
• Broaden applications for tissue forms
• Optimize donor criteria

6
Avenues for Research Tissue

• Internal MTF RD Projects
• MTF Research Tissue Program (Hospitals
  Universities)
• Application process
• MTF Research Grant Program
• Directed research
• Grant applications

7
Fresh Osteochondral Allografts

• Application Cartilage repair, eliminate pain and
  morbidity associated with autologous grafting
• Study Affect of storage media on cell viability
  of fresh refrigerated osteochondral allografts
  for articular cartilage repair
• Result Proprietary media developed to maintain
  cell viability, allowing introduction of living
  chondrocytes to the implant site

8
Acellular Dermis (Flex HD)

• Application Hernia repair, pelvic floor
  reconstruction, urological incontinence, rotator
  cuff repair
• Study Develop a process to remove epidermal and
  dermal cells from donated skin producing
  acellular dermis matrix
• Result Matrix supports cellular repopulation
  and vascularization at the surgical site.
  Supplied pre-hydrated stored at room temperature

9
Precision Spinal Allografts

• Application cervical fusions, lumbar fusions
  spinal corrections
• Study Develop machining methods to create
  intricate designs consistent sizing
• Result Spinal fusion grafts including technique
  specific precision shapes capable of restoring
  spinal function, enhancing patients lives
  eliminates donor site pain associated with
  autologous grafting

10
Bone Tendon-Bone Allograft

• Application ACL repair, eliminates pain
  associated with autologous grafting
• Study Collaboration with Dr. Charlie Gatt, MD -
  determine donor criteria for BTB grafts
• 100 Hemi BTB Grafts
• Age Range 16 69, sex matched
• Result No statistical difference between age
  and sex

11
AATB STAC Study on Fosamax

• Application MTF currently rejects donors
  treated with Fosamax
• Affect on bone transplantation unknown
• of donors treated with Fosamax continues to
  increase
• Effects both male and female donors
• Study 4 Major tissue banks providing research
  tissue (DBM) to test difference in quality
• Result Potentially broaden donor criteria
  increasing use of the gift

12
Non-standard Donors

• Application MTF donor age limit for weight
  bearing tissues is 70
• Study Evaluate differences in cortical bone
  from male donors 55-70 and 71-80 y/o
• Result Donors over 70 years of age are not
  acceptable for weight bearing applications

p lt 0.05
13
Research Tissue Program

• Prosthesis attachment for war veterans
• 100 femurs
• Development of fixation
• Development of a Device to Increase Proximal
  Femur Strength in Patients with Osteoporosis
• Hip fractures
• Method reinforcement with PMMA cement to prevent
  repeat injury
• Mechanical testing

14
Research Tissue Contd.

• Geometric and material properties of ACL, Patella
  Tendons and Hamstring Tendons
• ACL graft failure risk factors
• Male vs. female tissue
• Dimensions and mechanical testing

15
MTF Directed Research

• Effects of Aging on Bone
• Evaluation of Bone Density Through Radiographic
  Techniques
• Effects of Processing and Low Dose Radiation on
  Cortical Bone Grafts

16
MTF Grant Recipients

• Optimization of Novel Sterilization Methods for
  Soft Tissue AllograftsPI Michael Dunn, Ph.D. -
  UMDNJ-Robert Wood Johnson Medical SchoolAmount
  Funded 125,000
• Osteoprogenitor Cell Differentiation When
  Cultured on Allograft BonePI Stuart Goodman,
  M.D., Ph.D. - Stanford University
• Amount Funded 82,336
• Physiologic Loading for Long-Term Maintenance of
  Osteochondral Grafts in CulturePI Clark Hung,
  Ph.D. - Columbia University
• Amount Funded 125,000

17
Summary

• Research tissue plays an important role in our
  ability to
• Honor maximize the gift
• Provide highest quality and innovative allografts
• Support progression of external research
• Without research consent, non-transplantable
  donors can not be used

18
Thank You!

• Questions?