PowerPointPrsentation

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Percepción y Deconvolución
Dr. Steffen Härtel, CECS, Valdivia, Chile
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1 La percepción humana
2 Visión de máquinas (machine vision) en la
microscopía contemporánea
3 Ejemplos de aplicación en procesos
relacionados con la membrana celular
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1 La percepción humana
René Descartes (1596-1650) Epiphysis cerebri /
órgano pineal
... logramos una representación simbólica del
mundo exterior integrando información de...
1 señales directas de objetos
2 señales de otros sentidos
3 circuitos realimentativos
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2 Visión de máquinas (machine vision)
René Descartes (1596-1650) Epiphysis cerebri /
órgano pineal
NAOS Óptica Adaptativa
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Hans Janssen (1595),.... Galileo Galilei (1610)
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2Deconvolución
Objeto Original
Objeto Observado
Objeto Reconstruido
Deconvolución
Observación
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2Deconvolución
Objeto Original
Objeto Observado
Objeto Reconstruido
10 µm
Deconvolución
Observación
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2Deconvolución
Células Originales
Cortes a través de 2 células
Reconstrucción 3D
Deconvolución
Observación
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Hydrogen peroxide induced cell swelling in
necrotic HeLa cells. Absolute volume was
measured by reconstructing 3D confocal microcopy
stacks of DiIC18-stained HeLa cells as described
in Castro et al. (2005). A cluster of cells was
imaged before and during exposure to hydrogen
peroxide, which started at zero min. For each
time, the xy-projection is shown together with a
cross section in the xz-plane taken at the
indicated y-position (dotted line). Bars
represent 20 µm. Right Normalized volumes for
four experiments (0, 15, 30, 45 min) and two
experiments (-15 and 60 min). In each experiment
4 to 13 cells were measured. For 24 control cells
the volume measured was 4.0 1.0 pl. Context
Image processed confocal microscopy opens the
access to diverse structural, morphological, and
physiological features in cell biology in a
unique manner. The routines recently developed in
the scope of the project FONDECYT 3030065 permit
to determine time resolved changes in cellular
volume precisely on a single cell basis, and to
couple the data with novel molecular mechanisms
of ion transport during cell death. In the
presented context, the data published in Casto et
al. (2005) reveals that cell swelling occurs
before a delayed Ca2 overload in HeLa cells.

• La figura esta publicada en
• Castro, J., Ruminot, I., Porras, O., Flores, C,
  Hermosilla, T., Verdugo, E., Härtel, S. Barros,
  L.P. (2005) ATP steal a novel mechanism linking
  Na with the onset of necrotic Ca2 overload.
  revised version submitted to Cell Death
  Differentiation.
• La técnica de reconstrucción 3D de células a
  través de procesamiento de imágenes esta incluida
  en las siguientes presentaciones
• MORFOGÉNESIS ASIMÉTRICA DEL ÓRGANO PARAPINEAL EN
  EL CEREBRO EMBRIONARIO DE PEZ CEBRA. (Asymmetric
  morphogenesis of the parapineal organ in the
  embryonic zebrafish brain). Cabrejos M.E., Lemus
  C.G., Härtel S., Concha M.L. Programa de
  Anatomía y Biología del Desarrollo, ICBM,
  Facultad de Medicina, Universidad de Chile. CECS,
  Valdivia.
• CROSS CORRELATION AND SCALING INDEX METHODS FOR
  THE AUTOMATED QUANTIFICATION OF COLOCALIZATION IN
  FLUORESCENCE MICROSCOPY. Härtel1 S., Cornejo1 I.,
  Rojas2 R. y Barros1 L.F. 1Centro de Estudios
  Científicos, Arturo Prat 514, Valdivia, Chile.
  2Xperts Ltda., Yerbas Buenas 170, Valdivia,
  Chile.

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