500 bp

1
MgCl2 Titration of Titanium Taq (using optimized
50X Titanium Taq/10X Titanium Taq Buffer and calf
thymus gDNA template)
1.5
2.0
2.5
3.0
3.5
Mg (mM)
3.5
1.5
2.0
2.5
3.0
3.5
3.5
2000 bp
500 bp
2500 bp
900 bp
3500 bp
1500 bp
PCR performed using 10X Titanium Taq buffer
sent to Vendor M (already containing 3.5 mM
MgCl2). Only the 500 bp and 3500 bp primers were
utilized for this comparison.
2
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