The Big (Moldy) Enchilada (with beans)

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The Big (Moldy) Enchilada (with beans)

• Missie and Carrie
• With Carol Stevens
• At Seven Oak Middle School

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Our Students

• Two classes of 6th graders
• Approximately 55 students
• Variety of backgrounds and ability levels

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Objectives

• The students will be able to
• Understand and use the inquiry cycle
• Complete detailed procedures
• Compare and contrast lab and field sampling
  techniques

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Objectives, cont.

• Inoculate bean plants with germinated white mold
• Understand the role of genetics in agriculture
  and resistance

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Background Knowledge

• Introduction to the inquiry cycle.
• Exposure to experimental procedure writing.
• General knowledge of white mold.
• Bean Plants (soils lab).

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Week One Introduction to White Mold Lab

• Materials
• Inquiry cycle overhead

• Procedure
• Discussion of inquiry cycle
• Discussion of white mold
• Demonstration of procedure writing
• Procedure activity

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Take Home Messagefrom Week One

• Importance of accuracy
• in procedure writing
• (repeatability).

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Week Two Mold Sampling and Sterilization

• Materials
• Potato agar plates
• White mold samples with Sclerotia
• Agar plates of growing mold
• V8 juice
• Agar
• Distilled Water
• CaCO3

• Dr. Henrik Stotz, OSU CSS Dept. Presentation
• Field sampling
• Lab techniques
• Sterilization
• Making agar
• Agar inoculations

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Take Home Message from Week Two

• Importance of following
• procedures in sterilization
• Importance of sterilization
• in a lab

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Week Three Mold Inoculation

• Materials
• Plastic straws
• Bean plants (previously grown)
• Latex gloves
• Tweezers

• Procedure
• Demonstrate straw technique of inoculation
• Allow students to inoculate one leaf following
  procedure
• Students create experimental technique of
  inoculation

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Take Home Message from Week Three

• Repeatable procedures
• Development of experimental concept

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Week Four Genetic Resistance

• Materials
• None needed

• Procedure
• Discussion of observations on mold life cycle
• Discuss susceptibility to mold infection
• Genetic variability and resistance

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Week Four Unit Test

• Procedure writing
• Experimental error
• Sterilization techniques
• Mold and agriculture

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Questions?

• RSEP GK-12 Website http//cropandsoil.oregonstate
  .edu/gk12/index.htm
• Henrik Stotz e-mail
• stotzhe_at_science.oregonstate.edu