Bioinformatics

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Bioinformatics

• Day 1

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DNA is good

• DNA- Building blocks of life
• RNA- Portable copy of instructions
• PROTEIN- Tools and materials used for
  production

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Double Helix
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Four Bases

• Adenine
• Thymine
• Cytosine
• Guanine

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Synteny

• Same Gene Order
• Closely related
• No relation to Gene Simmons

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NCBI

• Steven Pechous
• Bethesda, MD

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Willem Rensink

• TIGR
• Genomics Technologies
• Plant Genomics
• Microbial Genomics
• Human Genetics

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ESTs

• Expressed sequence tags
• Single pass sequence from cDNA
• Specific tissue, stage, environment etc

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PCR

• Polymerase chain reaction
• Enzyme that doubles DNA

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Ted Kisha

• tkisha_at_wsu.edu
• National Plant Germplasm System
• Western Regional Plant Introduction Station

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Phenotypic

• Easy to read in the field
• Not affected by environment
• Cheap

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RFLPs

• Restriction Fragment Length Polymorphism
• Restricts entry of foreign DNA
• Detected by probing DNA

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AFLPs

• Amplified Fragment Length Polymorphism
• Cuts DNA with restriction enzymes

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Thanks Chuck