حسن رسولی. سانتریفیوژ

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• Just him

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Centrifuge

• By Hassan Rasouli
• M.Sc. Plant Biotechnology
• Supervisor Dr.K.Chaghamirza

Agriculture College in Razi University
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What is a Centrifuge?
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Actually

• Centrifuge is a piece of equipment, generally
  driven by a motor, that puts an object in
  rotation around a fixed axis, applying a force
  perpendicular to the axis.

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Centrifuge Works Principle

• The centrifuge works using the sedimentation
  principle.

Before the centrifuge
After the centrifuge
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Component main Centrifuge

• Rotors

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Type of Centrifuge

• A .Low-speed centrifuges
• Also called microfuge, Clinical, Table top or
  bench top centrifuges
• Max speed 20,000 rpm
• Operate at room temperature
• Fixed angle or swinging bucket can be used
• Commonly used for rapid separation of coarse
  particles
• E.g. RBC from blood, DNA from proteins, etc.
• The sample is centrifuged until the particles are
  tightly packed into pellet at the bottom of the
  tube. Liquid portion, supernatant, is decanted.

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• B. High-speed Centrifuges
• Preparative centrifuges.
• Max speed 80,000 rpm
• Often refrigerated, and requires vacuum to
  operate
• Fixed angle or swinging bucket can be used
• Generally used to separate macromolecules
  (proteins or nucleic acids) during purification
  or preparative work.
• Can be used to estimate sedimentation coefficient
  and MW

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• C. Ultracentrifuge
• The most advanced form of centrifuges
  (specialized and expensive)
• Used to precisely determine sedimentation
  coefficient and MW of molecules, Molecular
  shape,Protein-protein interactions
• Uses very high speed and/or RCF
• Uses small sample size (lt 1 ml)
• Uses relatively pure sample
• Built in optical system to analyze movements of
  molecules during centrifugation.

Analytical Ultracentrifuge
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Applied Centrifuge

• Segregate a material soluble.
• Segregate a DNA.
• Segregate a protein.
• Segregate a blood cell.
• Segregate a soluble small thing.
• And etc

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Danger!
Care and Attention!
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Truly yours