Phlebotomy- the collection of blood

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Phlebotomy- the collection of blood
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• In CA must be certified. ROP, various private
  schools offer phlebotomy certification.
• Starting salary 12-18/hr
• In the laboratory, easiest place to get hired,
  easiest place to get fired

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• http//library.med.utah.edu/WebPath/TUTORIAL/PHLEB
  /PHLEB.html
• An excellent tutorial on phlebotomy

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Draw Sites

• Finger sticks
• Median cubital vein (anticubital)
• Infants-heel stick
• Hand
• Any other sites, especially from the feet, should
  not be drawn without a physicians order
• Note never draw above an IV site

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• There are MANY different tubes that are used to
  collect blood. Each has a different additive
  (anticoagulant) and is used for specific tests.
  They are NOT interchangable.

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• Red Top
• ADDITIVE None
• USES Chemistries, Immunology and Serology, Blood
  Bank (Crossmatch)
• MODE OF ACTIONBlood clots, and the serum is
  separated by centrifugation

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• Purple Top
• USES Hematology (CBC) and Blood Bank
  (Crossmatch)
• ADDITIVEEDTA
• MODE OF ACTION Forms calcium salts to remove
  calcium
• requires full draw - invert 8 times to prevent
  clotting and platelet clumping

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• Green Top
• USES Chemistries
• ADDITIVEPlasma Separating Tube (PST) with
  Lithium heparin
• MODE OF ACTION Anticoagulates with lithium
  heparin Plasma is separated with PST gel at the
  bottom of the tube

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• Light Blue Top
• USES Coagulation tests full draw required
• ADDITIVESodium citrate
• MODE OF ACTION Forms calcium salts to remove
  calcium