Antibody formats VH scFv VL CH1 CL CH2 Fab Whole Antibody

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Antibody formats
VH
scFv
VL
CH1
CL
CH2
Fab
Whole Antibody
CH3
SS
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Phage display

• phage M13 gIII gene encodes pIII protein 5 copy
  protein on surface.
• Cloning a coding sequence in frame with the glll
  gene allows the expression of the encoded protein
  on the surface of the phage.
• The DNA sequence encoding the binding domain is
  contained in the phage particle.

Peptide or antibody fragment
gIII/pIII M13 genome
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