http://ccrf.skku.ac.kr

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http//ccrf.skku.ac.kr
GC ???? ?? ??? - ???? -
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Carrier Gas(????)
Gas Chromatography
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1. Moisture Trap 2. Oxygen Trap 3. Chemical Filter
Carrier Gas
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Carrier Gas(????)
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Carrier Gas(????)
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Syringe
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Syringe
Syringe ? ??? ???? 1. Syringe ?? ??? 1520 ???
?? ???? ??? 2. capillary column ? ???? ????
Syringe needle ?? plunger? ? ? ?? ?? ??
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Syringe

• 2. ??? ?? ? ????
• ???? ???? ??? ?? ?? ?? ??(????? ??? ???,
  needle?
• ??? ???? ???? ?)
• ??? ??? ???? ???? ??? solvent? 5 20? ?? ????
  ??
• ??? ?? ??? ???? ??? 6? ?? ?? ? ?? ??
• ?? ? ??? ?? ?? ????? needle tip? ??? ?? ????
  plunge?
• ??? ???? ????
• - ??? ???? ???? ????? ?? ? ?? ?? ?? plunger? ??
  ???.
• needle tip? ?? ?? ??? ??? ??? ????.
• ???? ????? ???? repeating adapter ? ???? ????.
  
• - ?? ? ???? ??? ?? ??? solvent ? ??? ? ??? ????.

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Syringe
4. Plunger ? ?? 1) Metal Plunger - Plunger
? ?? ?? ?? ??? ??. - Plunger? ?? ??? ???
???? ?? ???? ???? ??? ??. plunger ? ?????
??? ??? ??? sealing? ????. - ???? ?? ?? ?
plunger? ???? ??? ???. 2) Metal Plungers of
plunger in needle syringes - Plunger ?
??? ? ? cover nut? ???? ??. - ???? ?? ???
plunger ? ??? ??? ? ?????. - ??? ??? micro
volume syringe ? plunger ? needle? ??? ? ? ??
??? ???? ?? ??.
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Syringe
4. Plunger ? ?? 3) Teflon Tip Plungers (Gas
Tight Syringe) - ???? ???? ? plunger?
pumping ??? ??? ??. - gas tight ???? ????
plunger? ??? ????. - ???? ????? ???? ??
????? ????, ???? ???? 60 ºC ??? ???? 20
30 ?? ????.
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Syringe
5. Needle? ?? ? ? ???? ??? ?? ?? ???? ??
??? ?? ? ??. ? ? ???? ??? ??? ??? ???? ???
dead volume ? ??? ?? ???? ??? ?? ???
???? ?? ??? ??? ????? ??? ??? ?????
??.
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Injection Port
????? ?? ??? ?? ??

• ??? ???? ??
• Septum
• Injection port liner
• Gold seal (in case split/splitless Inlet)

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Injection Port - Septum
What is the Function The septum isolates the
sample flow path from the outside world. It must
provide a barrier that is readily penetrated by
the injector needle while maintaining internal
pressure without contaminating the analysis. They
are generally made of special high-temperature,
low-bleed silicone rubber formulations.
Why Replace Septa should be replaced regularly to
avoid leaks decomposition sample loss
reduced column or split vent flow ghost
peaks column degradation
How to Minimize Problems Avoid problems by
using within the recommended temperature
range changing regularly installing hand
tight using septum purge when available
using autoinjectors using sharp syringe needles
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Injection Port - Septum
Bleed and Temperature Optimized Septa (BTO)
Extended Temperature Range, Low-Bleed Maximum
Injection Port Temperature 400 ºC Virtually
eliminates injection-port sticking
Pre-conditioned packaged in glass to prevent
ontamination Ideal for use with
low-bleed, Mass Spec capillary columns
Long-Life Septa Pre-pierced for extended life
and reduced coring The preferred Septum for
Autosamplers Ideal for Overnight Runs Up to
400 Injections Per Septum injection Maximum
Injection Port Temperature 350 ºC Soft, 45
Durometer, Easy On Autosampler Needles
Advance Green Septa True Long-Life, High
Temperature Green Septum More Injections per
Septum Reduced Injection Port Sticking
Maximum Injection Port Temperature 350 ºC
Packaged in glass vials for high purity
Economical alternative to competitors Green
Septa
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Injection Port Septum
Septum??? ?
Contaminated Septa
Clean Septa
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Injection Port Ferrules
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Injection Port Ferrules
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Injection Port Liners
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Injection Port Liners
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Column
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Column
?? ??? ??? ??
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Column
Column ??? ??
Longer columns provide better resolution
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Column
?? ?? ??? ??? ??
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Column
??? ???? ??? ??? ??
??? ??
??? ????
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Oven
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Detector
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Detector-TCD

• ?? Filament? OFF
• TCD ???? ??? Column? ??
• Column? ??? ? Blank Nut? ??? ?
• ?? ???.
•   Reference Gas? 2030ml/min ?? ??
• ?.(Makeup Gas OFF)
• TCD ??? ??? 300? ?? ??? ??
• ? Conditioning ???

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Detector-FID

• ??? ??? ?? 150?????? ??.
• Chlorinated solvent? ??? ??? ???
• ? ??? ?????.
• ???? ???? ???? ??? ?? ??
• ???? ???? ??? ?????.
• ??? ????? ?? ??? ?? ???
• ?? ?? jet? ????.
• - Packed column 0.018 inch I.D
• - Capillary column 0.011 inch I.D

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Detector-NPD

• FID? ??? jet? collector? ????
• ??.
• Jet?? ?? alkali salt? ??? ??
• ??? ???? Bead? ??? ???.
• NPD??? flame? FID?? ???
• normal hydrocarbon? ???? ???
• ??.

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Detector-ECD

• ?????? ECD??? ?? ????
• ??? ?? ? ????? ?? ????
• ???? ??. (99.9995 ??)
• ??, ??, ?? ???? ?? ????
• ????? Linear range? ??? ? ??.
• ??? ???? ?? ?? recondition??.
• ECD? ??? ?? Signal?? ?? ??
• ????? ?? ?? ? ???? ??.

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Detector- ?- ECD

• Micro-ECD? ??
• 1/10 ?? ???? ??? ??? ??? ?? ????? cell ??? 10?
  ???
• ??? Anode ??? ???? anode? cell ??? ??? ?? ???
  ?? ??? ??
•  
• Capillary column insert ??? ???? ? ??? ???? ??
  ?? ??
•  
• Turbulent flow ??? ?? ?? ??
•  
• Linearity algorithm ?? ??? ??? ?? ??

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Detector- FPD

• ??? ??? ??? 300??????
• ???? ???.
• ?? ?? ???? CO2? ????
• heteroatom? ?? quenching? ???
• ?? ?? ??? light absorption? ??
• ??.
• ??? ??? ??? ???? ? ???
• ???.
• ??? ??? ??? ??? ??? ??.
• Sulfur? response? ???? ???
• ????? ????
• phosphorus response? ???? ??
• ? ????? ????.

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??? ?? ?? ??
http//ccrf.skku.ac.kr
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??? ?? ?? ??
http//ccrf.skku.ac.kr
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??? ?? ?? ??
http//ccrf.skku.ac.kr
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??? ?? ?? ??
http//ccrf.skku.ac.kr
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http//ccrf.skku.ac.kr
GC ???? ?? ??? - ???? -