New 1100 Series DAD SL and MWD SL

1

• 80Hz Data Acquisition for Ultra-fast LC

• New 1100 Series DAD SL and MWD SL

2
1100 Series DAD SL and MWD SL
The 1st Diode-array Detector designed for
Ultra-fast LC
Agilent 1100 Series Diode-array Detector SL
Agilent 1100 Series Multi-wavelength Detector SL
3
1100 Series DAD SL and MWD SLOverview

• Next Generation Electronics and Firmware provides
• 80Hz Data Acquisition of up to 8 Signals
• Up to 100 resolution gain in ultra-fast,
  quantitative LC
• 80Hz Full Spectral Data Acquisition (DAD SL only)
• for ultra-fast peak purity analysis and spectral
  conformation even for trace level compounds
• Improved Diode-Array Front-end Electronics
• for minimized noise (typical lt /- 6µAU ASTM)
• New Build-in Data Recovery Card
• for a data never lost insurance
• New RFID Tags for all Flow Cells and UV Lamp
• for unambiguous data traceability
• LAN on Board
• eliminates need for additional LAN interface
• Future proof design
• Build-in web-server, USB, PCMCIA (WLAN, Bluetooth)

4
1100 Series DAD SL and MWD SLOverview (contd)

• New Electronic Temperature Control (ETC)
• For maximum practical sensitivity by minimized
  baseline wander, especially under harsh and
  fluctuating ambient temperature and humidity
  conditions
• New standard flow cell
• for maximum practical sensitivity by minimized
  RI-sensitivity and dispersion
• Builds upon 1100 DAD Optical Design
• preserves features like programmable slit and
  dual lamp design for highest sensitivity from 190
  to 950nm
• Up to 20x Sensitivity from 400 950nm
• 40 ( 80) Sensitivity by 8 and 16nm slit
• Re-use spectral libraries of 1100 DAD A and B
• Confidence and robustness of a diode-array
  detector with more than 25,000 installations.

5
What is Ultra-fast LC?

• What is the Objective of Ultra-fast LC?
• Significant gains in productivity, while
  maintaining or increasing data quality
• Ultra-fast LC provides up to 10x gains in
  analysis speed while preserving
  or increasing Resolution, Sensitivity, Linearity,
  Precision and Robustness.
• Thereby, Ultra-fast LC ensures compliance with
  strictest (regulatory) performance requirements.
• 2. Uncompromised compatibility with existing
  methods.
• Run conventional LC methods without compromising
  performance
• Comply with todays and future requirements
• 3. Improved Data Security.
• Ultra-fast LC systems provide a new level of data
  security and traceability that prevents
  data losses and minimizes the risk of false data
  interpretation.

6
What is Ultra-fast LC?
How fast is Ultra-fast?

• Conventional LC Analysis Times 5.0 120
  min
• Fast LC Analysis Times 2.0 5.0 min
• Ultra-fast LC Analysis Times 0.2 2.0 min
• Gradient Time 0.2 1.5 min
• Cycle Times 0.5 2.5 min
• 50 Peak Width 0.1 1.0
  sec

7
What is Ultra-fast LC?

• Which 1100 Configuration do I need for Ultra-fast
  LC?
• 1100 Series Ultra-fast LC System
• 1100 Series Binary Pump for precise,
  high-pressure mixing
  gradient formation and low delay volume
• 1100 Series WPS for precise, high-speed
  injection with
  lowest delay volume and carry over
• 1100 Series TCC for precise, peltier-controlled
  
  high-temperature LC up to 80C
• 1100 Series DAD/MWD SL for highest
  chromatographic resolution by 80Hz
  data rate
• Zorbax RRHT 1.8um Columns
  for highest
  efficiency at high linear flows

8
Whats the Benefit of 80Hz Data Acquisition Rate?
Peak Width, Resolution and Peak Capacity in
Ultra-Fast LC
80Hz versus 20Hz 30 Peak Width 30
Resolution 40 Peak Capacity 70 Apparent
Column Efficiency 80Hz versus 10Hz 55 Peak
Width 90 Resolution 120 Peak Capacity
260 Apparent Column Efficiency

• Sample Phenone Test Mix
• Column Zorbax SB-C18, 4.6x30, 1.8um
• Gradient 50-100 ACN in 0.3min
• Flow cell 5ul

9
Whats the Benefit of 80Hz Data Acquisition Rate?
Peak Width, Resolution and Peak Capacity in
Ultra-Fast LC
80Hz versus 20Hz Data Rate 30 Peak
Width gt 40 Peak Capacity 30 Resolution
gt 70 Apparent Column Efficiency 80Hz versus
10Hz Data Rate 55 Peak Width gt 120 Peak
Capacity 90 Resolution gt 260 Apparent
Column Efficiency
10
Performance Requirements of Ultra-Fast LC

• Ultra-fast LC using the 1100 DAD SL provides
  Resolution and Peak Capacity gains of up to 100.
• But Can I still fulfill my (regulatory)
  performance requirements under ultra-fast LC
  conditions?
• Quantification of Side Products at 0.05 level ?
• RT Precision lt 0.5 ?
• Area Precision lt 1 ?
• Peak Purity Analysis at Trace Levels ?
• Spectral Conformation at Trace Levels ?

11
Sensitivity and Linearity in Ultra-Fast LC Can I
Simultaneously Quantity Main Compounds and Side
Products at 0.05 Level?
mAU
Is the detectors Dynamic Range large enough to
accurately and precisely quantify Main Compound
and Impurities simultaneously?
2000
Main Compound 2000mAU
1500
1000
Impurities
Impurities 1mAU
500
DMSO
0
0.5
1
1.5
2
2.5
min
12
Sensitivity and Linearity in Ultra-Fast LC Is
the Noise Low Enough for my Quantitative Analysis?
Peak-to-Peak Noise on 13ul Flow Cell
27 26
Note 50µAU Noise gives S/N 20 at 1mAU
(0.05 level)
ASTM Noise Specification 20 µAU Peak-to-Peak
(/- 10 µAU)

• Temperature 20C
• DAD 254nm,16nm, Ref 360, 80nm
• PW gt 0.1min (2.5Hz, 2sec RT)

13
Sensitivity and Linearity in Ultra-Fast LC Is
the Linear Range Large Enough for my Quantitative
Analysis?

• Linearity (Caffeine Sample)
• Deviation at 2.0AU
• 2.0 (Vis Lamp off)
• 2.5 (Vis Lamp on)
• 5 Deviation
• 2.5 AU (Vis Lamp off)
• 2.4 AU (Vis Lamp off)
• Specification
• 5 Deviation at 2.0 AU

14
Sensitivity and Linearity in Ultra-Fast LC
Reproducibility of Main Compounds at 2000mAU
(100 Level)

• Gradient 5070 B in 0.85min
• Column 4.6 x 50, 1.8um
• Injection 5ul of
• 550 µg/ml Nimodipin
• Flow Rate 4 ml/min
• Flow cell 13ul
• Data Rate 80Hz
• Slit 8nm

• Overlay of 10 analyses at 245nm
• RT Precision 0.067 RSD
• Area Precision 0.13 RSD

15
Sensitivity and Linearity in Ultra-Fast LC
Reproducibility of Impurities and Side Products
at Trace Level
Overlay of 10 analyses at 245 nm

• Column 4.6 x 50, 1.8um
• Gradient
  5070 B in 0.85 min
• Injection 5ul
• Flow Rate 4 ml/min
• Flow cell 13ul
• Data Rate 80Hz
• Slit 8nm

• Nifedipin
• A 2.5mAU (0.1 level)
• RT Precision 0.092 RSD

• Nifedipin degradation product
• A 0.5mAU (0.03 level)
• RT Precision 0.123 RSD

16
Sensitivity and Linearity in Ultra-Fast LC Can I
quantity Impurities and Side Products at 0.05
Level?
Nifedipin at trace levels Peak Width 0.63 sec

• Conditions
• Column 4.6 x 50, 1.8um
• Gradient 5070 B in 0.85 min
• Injection 5ul
• Flow Rate 4 ml/min
• Flow cell 13ul
• Data Rate 80Hz
• Slit 8nm
• Result
• Under ultra-fast LC conditions the DAD SL allows
  accurate quantitation of impurities and side
  products at levels smaller than 0.05 of the main
  compound(s).

2mAU 0.1 level S/N 50
1mAU 0.05 level S/N 25
Noise 40 µAU
0.5mAU 0.025 level S/N 12
17
Spectral Analysis in Ultra-Fast LCCan I do Peak
Purity and Spectral Conformation at Trace Levels?
Is the spectral quality obtained at trace level
under ultra-fast LC conditions and 80Hz spectral
sampling rate good enough for peak purity
analysis and spectral conformation?
18
Spectral Analysis in Ultra-Fast LCCan I do 80Hz
Peak Purity Analysis at Trace Levels?
Overlay of extracted Nifedipin spectra at trace
level
UV Spectrum of Nifedipin at higher concentration
(ca. x180)
Result Nifedipin peak at 0.1 level (1.8mAU)
measured with 80Hz spectral rate is pure no
other compounds are co-eluting with Nifedipin
19
Spectral Analysis in Ultra-Fast LCCan I do 80Hz
Spectral Conformation at Trace Levels?
Norm
Norm
2
2

• Overlay of 80Hz Reference and Apex Spectrum
• Nifedipin Ref 1800mAU (100 level)
• Nifedipin Apex 1.8mAU (0.1 level)

• Overlay of 80Hz Reference and Apex Spectrum
• Nimodipin Ref 1800mAU (100 level)
• Nifedipin Apex 1.8mAU (0.1 level)

1.75
1.75
1.5
1.5
1.25
1.25
Match Factor 963
Match Factor 929
1
1
0.75
0.75
0.5
0.5
0.25
0.25
0
0
nm
300
400
500
600
700
800
900
nm
300
400
500
600
700
800
900
Result Identification of Nifedipin at 0.1
trace level under fast LC conditions and 80Hz
spectral sampling rate
20
Speed and Precision in Ultra-Fast LCPushing the
Limits for highest Throughput
Overlay of 6 Runs

• Performance
• Average 50 Peakwidth 0.34 sec
• Resolution (4,5) 1.5
• Analysis Time 24 sec
• Cycle time 50 sec
• RT Precision 0.7 0.22 RSD
• Area Precision 1.5 0.3 RSD

• Conditions An extreme Example
• Sample Phenone Test Mix
• Column 4.6 x 30mm, 3.5µm SB-C18
• Gradient 50-100 ACN in 0.3min
• Flow rate 5ml/min
• Temperature 40C
• Data Rate 40Hz

• Application Areas
• Screening Experiments
• HT LC/MS/UV
• Early Formulation Studies
• Process Analytical Techn.

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Speed and Precision in Ultra-Fast LCModerate
Gradients for highest-quality Quantitative Data

• Overlay of 6 Runs
• 2x slower gradient
• 2 5x better Precision
• Still very fast

• Performance
• Peakwidth 0.38 sec FWHM
• Resolution (4,5) 1.6
• Analysis Time 40 sec
• Cycle time 70 sec
• RT Precision 0.1 0.5 RSD
• Area Precision 0.2 0.5 RSD

• Conditions Less extreme Condtions
• Sample Phenone Test Mix
• Column 4.6 x 30mm, 3.5µm SB-C18
• Gradient 50-100 ACN in 0.6min
• Flow rate 5ml/min
• Temperature 40C
• Data Rate 40Hz

• Application Areas
• Formulation Studies
• Analytical Development
• Process Control
• QA/QC

22
Robustness in Ultra-Fast LCAre Methods and
Instrumentation Robust Enough for 24x7 Operation?
Column 1
P300bar
injection 4000
injection 2000
injection 1
Column 2
injection 4000
injection 2000
injection 1

• Stability study on system configuration with
  automated column regeneration
• Stable system and column performance for 8000
  injection (4000 injections per column)
• System suitable for unattended and automated 24x7
  operation and reliable over-weekend runs

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New Data Recovery CardThe first LC Detector
with Data Never Lost Insurance

• Data Recovery Card - DRC
• All signals, spectra and meta data are buffered
  on high-capacity, embedded 256MB Compact Flash
  Card compliant with 21 CFR Part 11.
• Prevents any data loss in case of communication
  breakdowns between instrument and PC.
• Automatic Run Recovery in case of temporary
  communication failures
• Manual Run Recovery in case of permanent
  communication failures after software, PC,
  and/or instrument re-boot.

Patent Filed
24
New Data Recovery CardThe first LC Detector
with Data Never Lost Insurance
Automated Run Recovery in case of temporary
communication failures
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New Data Recovery CardThe first LC Detector
with Data Never Lost Insurance
Manual Run Recovery in case of permanent
communication failures

• Run Recovery dialog pops-up automatically after
  system re-boot.
• Data are stored on the PC under a pre-configured
  location.

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The Next Level of Data Traceability Proprietary
RFID Technology for Flow Cells and UV Lamp

• Radio Frequency Identification Tags
• RFID tags records all relevant data necessary to
  recall instrument conditions under which a run
  has been executed.
• Minimizes the risk of false data interpretation,
  because measurement conditions are documented.
• Meta data stored on RFID tags are saved with each
  raw data file for unambiguous answers to
  (auditor-) questions like
• Which type of flow cell was used to generate
  this chromatogram - what was the path length and
  volume?
• Did the accumulated burn-time of the lamp
  exceed pre-defined limit?

• Flow Cell
• Path length
• Volume
• Max pressure
• Date last test passed
• Product number
• Serial number
• Production date

• UV Lamp
• Accumulated on-time
• Actual on-time
• Number of ignitions
• Date last test passed
• Product number
• Serial number
• Production date

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The Next Level of Data Traceability Proprietary
RFID Technology for Flow Cells and UV Lamp
ChemStation Report RFID-tag information documents
run conditions.
28
The Next Level of Data Traceability Proprietary
RFID Technology for Flow Cells and UV Lamp
RFID Tag Info for Diagnostics
29
The next Level of Baseline StabilityNew
Electronic Temperature Control ETC
Electronic Temperature Control - ETC

• Advantages of ETC
• Compensation of changes of ambient conditions
  (temperature and humidity)
• Reduced baseline wander for improved practical
  sensitivity and reproducibility under harsh
  environmental conditions

Ambient Temp. Sensor
Optical Temp. Sensor
Fan
Heater
Air Flow
Optical Unit
Main Board
Front
1100 Series DAD SL / MWD SL
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The next Level of Baseline StabilityNew
Electronic Temperature Control ETC
TempCntrl ON 254,4 No Ref 750,4 No Ref
TempCntrl OFF 254,4 No Ref 750,4 No Ref
Ambient Rejection at 60 RH ETC off
700µAU/C ETC on lt 30µAU/C
2.6mAU / 4C 0.7mAU/C
Conditions Relative humidity 60RH const
Temp 25C /-2C 4 x 1h Cycles Note By
keeping RHconst, the absolute humidity is
strongly modulated due to temperature variations
(worst case condition).
31
The next Level of Baseline StabilityNew
Electronic Temperature Control ETC
0.5mAU/C
Ambient Rejection at 95 RH ETC off
700µAU/C ETC on lt 30µAU/C
0.7mAU/C
Conditions Relative humidity 95 RH const
Temp 25C /-2C 4 x 1h Cycles Note By
keeping RHconst, the absolute humidity is
strongly modulated due to temperature variations
(worst case condition).
32
The next Level of Baseline StabilityNew
Electronic Temperature Control ETC
Comparison between 1100 DAD SL and 1100 DAD
(B-model)
Ambient Rejection ETC on DAD B
100µAU/C DAD SL lt 30µAU/C
1100 DAD SL 254,4 / No REF
1100 DAD SL 254,4 / 360,100
100µAU/C
1100 DAD B 254,4 No REF
1100 DAD B 254,4 / 360,100
Conditions Relative humidity 60RH const
Temp 25C /-2C 4 x 1h Cycles Note By
keeping RHconst, the absolute humidity is
strongly modulated due to temperature variations
(worst case condition).
33
New 13ul Standard Flow Cell Design
Ceramic Ring for thermal de-coupling

• Advantages
• Reduced RI-sensitivity
• Improved peak dispersion
• Minimized noise in in high-flow, high-temperature
  applications

• Insert- Body- Ring

Drill Design of Inlet and Outlet for faster
flush-out
Patent Filed
34
New 13ul Standard Flow Cell Reduced RI
Sensitivity
Demanding RI Test Gradient
36 mAU
12 mAU
9 mAU
4 mAU
New Standard Flow Cell provides 3-4x lower
RI-Sensitivity
1100 DAD/MWD with old Standard
Cell 1100 DAD SL/MWD SL with new Standard Cell
35
New 13ul Standard Flow CellMinimized Dispersion
for Maximum Resolution and Sensitivity
Improved flush-out behavior of the new standard
flow cell minimizes peak tailing thereby
increasing peak heights and resolution.
Column Sample Temp Flow WL Eluent Injection
SB-C18 1.0x50 Iso Std H2NCSNH2 25 0.1
ml/min 254,4 -/- 80/20 H2O/ACN 1 ul
new Standard Cell old Standard Cell
36
Flow Cell Portfolio of the 1100 DAD/MWD SL For
Uncompromised Compatibility from Nano to Prep

• Advantages From Nano to Prep
• Compatibility with Conventional LC, Ultra-fast
  LC, Capillary LC, Nano LC and Prep LC
• Support of Analytical LC on Columns from 75 µm to
  4.6mm ID
• Support of Preparative LC on Columns from 4.6 to
  50mm ID
• RFID tags for Data Traceability and Diagnostics

• Flow Cell Portfolio
• Standard 13ul, 10mm path length, 120bar
• Semi-Micro 5ul, 6mm path length, 120bar
• Micro 1.7ul, 6mm path length, 400bar
• Semi-Nano 500nl, 10mm path length, 50bar
• Nano 80nl, 6mm path length, 50bar
• Preparative 3mm, 120bar
• Preparative 0.3mm, 20bar
• Preparative 0.06mm, 20bar

• Insert- Body- Ring

37
Which Flow Cell to use for Ultra-fast LC?

• 13µl Standard Flow Cell
• For highest sensitivity and linearity
• High-demanding quantitative work, e.g.
  analytical method development, QA/QC
• 4.6 3 mm ID Columns
• 1.7µl Micro Flow Cell
• For highest selectivity
• Ultra-fast semi-quantitative work,
  e.g. Screening Experiments, HT
  LC/MS/UV
• 2.1 1mm ID Columns
• 5µl Micro Flow Cell
• Best compromise of sensitivity and selectivity
• For good quantitative and qualitative results,
  e.g. Screening, HT LC/MS/UV, Early Formulation
  Studies
• 4.6 1mm ID Columns

Depends on analytical conditions and column
dimension
38
Which Flow Cell to use for Ultra-fast LC? Noise
Comparison
5ul Semi-Micro Flow Cell Noise lt /- 2.4 uAU
Note 5ul cell shows similar noise as 13ul cell.
However, the linear range is reduced due to the
reduced path length of 6mm.
Specification ASTM noise lt /- 10 uAU

• Conditions
• Column 4.6x30mm SB C18, 1.8um
• Flow rate 1ml/min
• Mobile phase Water
• Temperature 20C
• DAD 254nm, 16nm, Ref 360, 80nm
• PW gt 0.1min (2.5Hz, 2sec RT)
• Slit 16

13ul Standard Flow Cell Noise lt /- 2.1 uAU
39
SensitivityLimit of Detection for Anthracene
under Ultra-fast LC Conditions
Performance LOD/pg in Ultra-fast LC Retention
Time 12 sec, Peakwidth 0.9 sec
27 26
Compare Conventional LC on 1100 DAD/MWD B
Retention Time 2 min, Peak width 6 sec
40
1100 Series DAD SL and MWD SL Builds upon 1100
DAD Optical Design - Optimized for Best
Sensitivity
1100 DAD 25,000 Installations Worldwide
Tungsten lamp
Long-life Deuterium Lamp
Excellent wavelength resolution
1024 element diode array
Holmium Oxide Filter
950 nm
Flow Cell
190 nm
Minimized Noise in Visible WL-Range
More uptime gt 2000h
Programmable slit
Automated wavelength verification
Grating
Fast optimization of sensitivity and resolution
41
1100 Series DAD SL and MWD SL Builds upon 1100
DAD Dual Lamp Design for Highest Sensitivity
from 190 to 950nm
Tungsten Lamp Off Noise lt 20 µAU
254nm

• Advantages
• Approx. constant Noise from 190 to 950nm
• Up to 20x lower Limits of Detection for compounds
  absorbing in the visible range at ? gt 400nm
• Significantly higher confidence in qualitative,
  spectral analysis results
• More accurate Peak Purity results, especially at
  trace levels.
• More accurate Library Analysis and Spectral
  Confirmation results

Tungsten Lamp On Noise lt 20 µAU
Tungsten Lamp Off Noise gt 400 µAU
700nm
Tungsten Lamp On Noise lt 20 µAU
42
1100 Series DAD SL and MWD SL Builds upon 1100
DAD Programmable Slit Micromechanics

• 1 Programmable Slit for 1, 2, 4, 8 and 16 nm
• Advantages
• No need for manual slit changes
• Fast optimization of sensitivity and resolution
• Maximized Sensitivity by 16nm slit
  ( 80 versus 4nm slit)
• Maximized Spectral Resolution by 1nm slit (for
  optimized spectral analysis)
• Documents slit width (GLP, data traceability)

Motor
Slit
43
1100 Series DAD SL and MWD SL Builds upon 1100
DAD Programmable Slit for fast Optimization of
Sensitivity and Resolution
Absorbance
Resolution Benzene Spectrum at Trace Level 0.7
mAU 1 nm slit 2 nm wavelength bunching
nm
230
240
250
260
270
280
1 nm
Noise level
2 nm
4 nm
8 nm
16 nm
44
Rapid Resolution HT 1100 Series Modification
KitsConverting an 1100 Binary System to
Ultra-fast LC

• 3 Modification Kits
• 1100 VWD 4.6mm RRHT-1100 Series Ultra-fast LC
  Kit P/N 5188-5323
• 1100 DAD 4.6mm RRHT-1100 Series Ultra-fast LC
  Kit P/N 5188-5324
• 1100 DAD/MS 2.1mm RRHT-1100 Series Ultra-fast
  LC Kit P/N 5188-5328

• Content
• Filter
• Capillaries
• Fittings, Union
• UV Flow cell
• RRHT Columns

45
Rapid Resolution HT 1100 Series Modification
KitsConverting an 1100 Binary System to
Ultra-fast LC
Fast LC Modifications for 1110 Binary LC System
with DAD/MWD Detector and 4.6mm ID RRHT Columns
46
Summary1100 Series DAD SL and MWD SL

• 80Hz Data Acquisition
• for up to 100 resolution gain in ultra-fast LC
• High Precision, Linearity and Sensitivity
• to maintain data quality under ultra-fast LC
  conditions
• to comply with regulatory requirements
• to allow for spectral analysis at trace levels
  (DAD SL only)
• High Instrument, Column and Method Stability
• enables robust 24x7 operation
• Uncompromised Compatibility with Existing Methods
• Run conventional methods without compromising
  data quality
• Build-in Data Recovery Card
• provides data never lost insurance
• RFID Tags for Cells and UV Lamp
• for unambiguous data traceability
• New Low Noise Electronics, New ETC, New Standard
  Flow Cell
• for decreased short-term noise and increased
  practical sensitivity

47
Appendix
48
Overview Features and Specifications
Note For uncompromised compatibility from
nano-flow to preparative applications all
detectors can be ordered with 5 different
analytical flow cells (13ul, 5ul, 1.7ul, 500nl,
80nl) and 3 preparative flow cells (3mm, 0.3mm
and 0.06mm)
49
New Electronics platform
Module Independent Electronic Core

• Future proof design
• State-of-the-Art Motorola PowerPC Processor
• LAN on board
• USB on board
• PCMCIA
• Firewire
• WLAN
• Bluetooth
• Integrated Web-server

50
Peak Width Response Time Data Rate and
Sensitivity
Dont use for gt 0.15 sec peak width.
gt 0.15 sec gt 0.3 sec gt 0.6 sec gt 1.2 sec gt 3
sec gt 6 sec gt 12 sec gt 24 sec gt 51 sec
Recommended settings in ultra-fast LC with
50 peak width between 0.15 and 0.6 sec
For 50 peak width between 0.6 and 1.2 sec

• Notes
• Noise level changes proportional to the square
  root of the change in data rate.
• For optimum selectivity and sensitivity the Peak
  Width should not be chosen smaller than
  necessary.
• For 50 peak width between 0.3 and 0.6 seconds
  Peak Width of gt 0.005 min is recommended, which
  correspondes to 40Hz data rate.
• For peaks narrower than 0.3sec at half height,
  Peak Width of gt 0.0025min (80Hz data rate) should
  be used.
• For highest sensitivity in ultra-fast LC the slit
  can be increased to 8 or 16nm.

Peak Width Peak Width at 50 Peak Height
51
Linearity - OQ/PV Test on Caffeine Standards
Correlation 1.00000
52
Agilent 1100 Series HT LC/MS SystemA Scalable
and Flexible Solution for Ultra-fast Analysis
1.
3.
4.
5.
2.
6.

• HT LC System 1.8um RRHT Columns
• Capacity Extension
• Valves

• Mass-selective Detector
• Integrated Controller
• Services and Compliance Products

53
Van Deemter Curves1.8um Rapid Resolution HT
Columns on the 1100 HT System
Higher speed Higher resolution Higher
sensitivity
Particle H_min 5µm 9.3µm 3.5µm 6.0µm 1.8µm 3.8µm
Note Efficiency of 1.8µm columns is virtually
flow-rate independent.
ZORBAX Eclipse XDB-C18 4.6 x 50mm (30mm) 8515
ACNWater 1.0?L Octanophenone 0.05 5.0
mL/min 20C
5.0 ?m
HETP (cm/plate)
Efficiency gain of 1.8µm versus 5µm columns
3.2x _at_ 2ml/min 4.4x _at_ 5ml/min
3.5 ?m
1.8 ?m

• Up to 2.1x Resolution
• Up to 4.4x Speed

260,740 N/m _at_ 2mL/min
5.0 mL/min
Interstitial linear velocity (ue- cm/sec)
54
Ultra-fast Gradient Analysisof 9
PhenonesPerformance Summary

• 9 Phenones baseline separated in 29 sec
• 39 sec
• 0.8 min (with column regeneration and run time
  0.7min)
• 1.0 min (without column regeneration and run time
  0.7min)
• gt 2.65 for all peaks
• 0.50 sec (average)
• 79 (0.65min gradient)
• lt 0.2 RSD without column regeneration
• lt 1.0 RSD with column regeneration
• lt 0.003 (limit of detection)
• 7.2 days or 8000 injections of continuous
  operation with stable performance

Analysis speed Analysis time Cycle
time Resolution 4? peakwidth Peak capacity RT
precision Carry over Robustness