Folie 1

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Detoxophane Activating the skins detoxification
system
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Mom was right Eat your vegetables, they are
good for you!

• Phytonutrients are plant chemicals reported to
  exert a specific effect on health or on
  amelioration of diseases
• Health benefits of vegetables Lower risk for
  certain cancers, stroke, and high blood pressure

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The Vegetables of the Mustard Family

• Broccoli sprouts are now extreme popular as food
  supplements in the USA
• Vegetables of the mustard (Brassicaceae) family
  have been shown to be extremely healthy
• They contain a high concentration of sulforaphane
• Members of the Brassicaceae vegetables are
  broccoli, cauliflower, radishe, water and garden
  cress

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Garden Cress Sprouts The Source for a New
Cosmetic Ingredient

• Garden cress is a suitable form for sulforaphane
  for cosmetics
• Fresh sprouts are homogenized and extracted with
  water
• The extract is purified, concentrated and
  encapsulated into liposomes

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Composition

• Lepidium Sativum Sprout Extract
• Glycerin
• Lecithin
• Phenoxyethanol
• Aqua/Water

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Isothiocyanates Molecular Structures
In the plant, isothiocyanates are present as
glucose derivatives, called glucosinolates. When
the plant cells are broken, the enzyme myrosinase
is liberated. This thioglucosidase transforms
glucosinolates into free glucose and
isothiocyanates.
Sulforaphane
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Sulforaphane Mechanism of Action

• Sulforaphane activates the bodys detoxification
  system composed of phase I and phase II enzymes
• Phase I reactions increase the reactivity of
  hydrophobic molecules by addition of functional
  polar groups (-gtreactive oxygen species). If
  these reactive molecules are not further
  metabolized by phase II conjugation, they may
  cause damage to DNA and induce mutations.
• Phase II reactions are either conjugation
  reactions of phase I products with small
  hydrophilic substances to form water-soluble
  products that can be excreted or they are
  antioxidant enzyme reactions that inhibit strong
  oxidant formation.

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Detoxophane is More than an Antioxidant
UV
ROS
Antioxidant
Detoxophane
Lipidperoxides
Toxin
AGE
DNA damage
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Activation of Gen Expression with the
Antioxidant Response Element (ARE)

• The antioxidant response element ARE is a
  regulatory promoter sequence of genes coding for
  antioxidant systems and phase II detoxification
  enzymes
• The transcription factor Nrf2 is the critical
  regulator of ARE-dependent gen expression
• Under normal conditions Nrf2 is blocked by the
  repressor Keap1
• Sulforaphane was found to disrupt the Nrf2/ Keap1
  complex

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Detoxophane The Next Generation Antioxidant

• Stimulates the expression of antioxidants and
  detoxification enzymes in the skin to protect
  against
• Environmental pollutants (cigarette smoke,
  exhaust fumes, smog, endotoxins, heavy metals)
• Reactive oxygen species (extrinsic and intrinsic)
• DNA damage and thus premature skin aging

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Modulation of Enzyme Expression

• Method
• Human keratinocytes were grown to 80 confluence
  in a standard growth medium.
• Then cells were incubated during 24 hours with
  different concentrations of Detoxophane.
• Cells were harvested and total RNA extracted.
• Real time quantitative PCR was used to analyze
  gene expression
• Phase I - Cytochrome P450 isoenzyme 2E1 (CYP2E1)
  
• Phase II - NADPH quinone reductase 1 (NQO1)
• - Heme oxygenase 1 (HO-1)
• - Thioredoxin reductase 1 (TrxR1)

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Modulation of Enzyme Expression
S-131
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Protection Against Oxidative Stressors

• Method
• Growth of human keratinocytes to 80 confluence
  in a standard growth medium
• Pre-treatment for 24 hours with 0.05 Detoxophane
• Incubation for 4 hours with increasing
  concentrations of toxins
• - 4-hydroxynonenal ? HNE
• - tert-butyl hydroperoxide ? t-BH
• Analysis of cell viability with MTT assay

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Protection Against Tert-Butyl Hydroperoxide
S-148
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Protection Against Tert-Butyl Hydroperoxide

• Detoxophane exerted an impressive protection
  against t-BH. At 0.05 Detoxophane, the IC50
  value increased from 258 microM of the control to
  646 microM.
• Detoxophane exerted a moderate protection against
  HNE. At 0.05 Detoxophane, the IC50 value
  increased from 76 microM of the control to 90
  microM.

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Summary

• Vegetables of the mustard family, such as
  broccoli and garden cress sprouts, are known for
  their remarkable health benefits
• The phytonutrient sulforaphane has been
  identified as the active compound
• Garden cress sprouts have been used to develop
  Detoxophane as cosmetic sulforaphane preparation

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Claims

• Detoxophane
• Activates the detoxification system of skin cells
• Protects skin against pollutants
• Prevents visible signs of skin aging
• Protects against city pollution
• Prevents DNA damage
• Reveals a healthy looking skin

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Application

• Anti-Ageing Formulations
• Detox City Creams
• Sun Protection Formulations
• Hand Creams
• Protective Foundations

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Film