Culturing Yeast Cells on Media

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Culturing Yeast Cells on Media
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• Pre Lab Definitions
• Petri Dish A round, shallow dish used to grow
  bacteria.
• Culture To grow living organisms in a prepared
  mixture of nutrients (media).
• Media Substance containing nutrients needed for
  cell growth.
• Agar Jelly to which food has been added for the
  growth of microbes.

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• Pre Lab Definitions
• Inoculate To introduce a microbe to an
  environment where it can grow.
• Sterile/Aseptic Technique Laboratory procedures
  used in handling cultures, media and equipment
  that prevent contamination.
• Colony A visible cluster or group of cells
  growing on a solid medium.

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• Pre Lab Definitions
• Safety Equipment
• Lab Coat, Glasses, Gloves

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Lab- Part 1 Serial Dilution of Yeast Broth

• 1. Label test tubes 1, 2, 3, 4.
• 2. Add 9 mL of water to test tubes 1, 2, 3, 4.
• 4. Add 1 mL (20 drops) of yeast broth (from the
  shake flask) to test tube 1. (mix)
• 5. Pipette 1 mL of solution from test tube 1 to
  test tube 2. (mix)
• 6. Pipette 1 mL of solution from test tube 2 to
  test tube 3. (mix)

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Lab- Part 1 Serial Dilution of Yeast Broth

• 7. Pipette 1 mL of solution from test tube 3 to
  test tube 4. (mix)

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Lab Part 2- Inoculation of media with Yeast Cells

• 1. Label your petri dish with your groups name
  and number (on the underside of the dish).
• 2. Swirl your disposable loop in test tube 4.
• 3. Use the disposable loop to spread the solution
  on the surface of the agar- being careful not to
  press too hard!
• 4. Place your dish in the incubator.
• 5. The cells will incubate or grow at 30 degrees
  C for 24 hours.