InVitroCare SpermCare One-Step IUI System

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InVitroCare SpermCare One-Step IUI System
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InVitroCare SpermCare One-Step IUI System

• InVitroCare SpermCare One-Step is a ready to use
  sperm preparation system for Intrauterine
  Insemination (IUI). The system includes
  pre-measured gradient in 15mL centrifuge tubes
  and pre-measured InVitroCare Sperm Wash Medium in
  glass vials.
• The gradient, consisting of silane coated
  colloidal silica particles suspended in
  HEPES-buffered HTF, removes bacteria, epithelial
  cells, cell debris, immature sperm, and
  lymphocytes.
• All system components contain glucose as a
  useable energy substrate to maintain normal cell
  metabolism and sperm function.

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InVitroCare SpermCare One-Step IUI System

• Materials Included

• Materials Not Included

• 5 x 1 mL SpermCare One-Step
• (blue cap centrifuge tube)
• 5 x 2 mL InVitroCare Sperm Wash Medium
• (green cap vial)
• 5 x 0.75 mL InVitroCare Sperm Wash Medium
• (silver cap vial)
• 5 sterile transfer pipettes

• Centrifuge (must be able to operate for up to 30
  minutes at 400 g)
• Intrauterine insemination (IUI) catheters

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InVitroCare SpermCare One-Step IUI System

• Instructions for Use
• Bring all components of the system and semen
  sample to room temperature before use.
• Step 1 ADD SEMEN
• Gently layer 1-2 mL semen (at room temperature)
  on top of pre-measured SpermCare solution in blue
  cap 15 mL centrifuge tube.

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InVitroCare SpermCare One-Step IUI System

• Instructions for Use
• Step 2 SPIN
• Centrifuge at approximately 2000 rpm (300-400 x
  g) for 30 minutes.
• calculation of g force
• g1.118 rrpm²
• rradius of centrifuge in mm
• rpmrotations per minute/1000

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InVitroCare SpermCare One-Step IUI System

• Instructions for Use
• Step 3 WASH
• 3.1 Remove liquid layer, being careful not to
  disturb sperm pellet in bottom of centrifuge
  tube.
• 3.2 Re-suspend sperm pellet by transferring the
  contents of green cap vial (InVitroCare Sperm
  Wash Medium) into centrifuge tube.
• 3.3 Gently agitate.
• 3.4 Centrifuge at approximately 2000 rpm (300-400
  x g) for 5 to 10 minutes.
• calculation of g force
• g1.118 rrpm²
• rradius of centrifuge in mm
• rpmrotations per minute/1000

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InVitroCare SpermCare One-Step IUI System

• Instructions for Use
• Step 4 LOAD CATHETER
• 4.1 Remove liquid layer, being careful not to
  disturb sperm pellet in bottom of centrifuge
  tube.
• 4.2 Re-suspend sperm pellet by transferring the
  contents of silver cap vial (InVitroCare Sperm
  Wash Medium) into centrifuge tube.
• 4.3 Gently agitate.
• 4.4 Load entire volume (about 0.5-0.75 mL) of
  re-suspended sperm sample into an approved IUI
  catheter for subsequent transfer of the sperm to
  the uterine cavity.

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InVitroCare SpermCare One-Step IUI System

• Storage and Conservation

• Precautions and Warnings

• Store reagents at 2-8C when not in use. Open and
  close bottles under aseptic conditions. Contents
  cannot be re-sterilized after opening. Centrifuge
  tube and vials are designed for single use.

• All human, organic material should be considered
  potentially infectious. Handle all specimens as
  if capable of transmitting HIV or Hepatitis.
  Always wear protective clothing when handling
  specimens.

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InVitroCare SpermCare One-Step IUI System

• To place your order for InVitroCare SpermCare
  One-Step IUI System,
• Call 859-278-6806
• Email sales_at_zdlinc.com
• Website www.zdlinc.net