Module 1C

1
Module 1C
06/2007
UNMC General Biosafety Training Safety Equipment
2
General BiosafetyModule 1 Outline

• Overview
• Module 1A
• Biosafety Levels 1 and 2
• Module 1B
• Safety Equipment
• Module 1C
• Decontamination and Biohazardous Waste Handling
• Module 1D
• Biosecurity
• Module 1E

3
General BiosafetyModule 1C Synopsis

• Information about this training module
• For a comment or to ask a question, you will find
  a link on the main page of the course.
• To enhance the size of this PowerPoint
  presentation, click on the screen icon at the
  bottom right of this page
• Once this section is completed, click on the X
  and the top right of the window to close it and
  return to the main page to access Module 1D.

4
Who needs this training?

• Individuals who work with or have access to
  biohazardous materials.

5
Biological Safety Cabinets
6
Biological Safety Cabinets Overview

• Protection of
• Product
• Personal
• Environment

7
Biological Safety Cabinet Types

• Class II
• Type A1
• Air circulation
• 70 of air recirculated within the cabinet
• 30 exhausted through the HEPA filter back into
  the laboratory
• Not suitable for work with.
• volatile toxic chemicals
• volatile radionuclides
• Type A2 (formerly B3)
• Air circulation
• Similar to A1 with a higher face velocity
• May either be exhausted through.
• an exhaust canopy that is surrounded by negative
  pressured ducts and plenums (also called thimble
  connection) or
• exhausted into the room without connection to the
  exhaust system
• Hoods with a canopy may be may be used for work
  with
• minute quantities of volatile toxic chemicals
• tracer amounts of radionuclides

8
Biological Safety Cabinet Types
Class II - continued

• Type B1
• Air circulation
• 30 of air re-circulated
• 70 exhausted through the HEPA filter to the
  outside via a hard duct
• Hoods may be used for work with
• large amounts of volatiles (BSC/fume hood
  combinations)
• Type B2
• Air circulation
• No recirculation
• Total exhaust through the HEPA filter to the
  outside via a hard duct

9
Biological Safety Cabinet HEPA Filter

• High efficiency particulate air filter
• Traps particulates only
• chemicals, fumes, and vapors pass through
• Traps particulates 0.01 microns and larger
• includes bacteria and viruses

10
Biological Safety Cabinet HEPA Filter

• Filter construction
• Metal or wood framed
• Continuous sheet of flat filter glass medium
• with aluminum separators
• Gasket sealed
• Adhesive bonded between filter pack and frame
• Have an indefinite lifetime under general use

11
Biological Safety Cabinet Airflow
Exhaust
Typical Class II
Intake 100 ft/min
12
Biological Safety Cabinet Operation
1. Load with needed supplies 2. Turn on and allow
to run for 10-15 m 3. Check inward airflow with a
piece of tissue 4. Enter straight into cabinet
and perform work in a slow, methodical
manner 5. At end of work package or decontaminate
items to be taken out 6. Decontaminate
interior 7. Allow cabinet to run for 10-15
m 8. Shut off
13
Biological Safety Cabinet Operation

• Always enter straight into cabinet
• - no sweeping motions
• Place materials well within the cabinet
• -not on the front grill
• Place discard pan within cabinet
• Watch for disruptions of air flow
• Package biohazardous materials before removal
  from cabinet

14
Biological Safety Cabinet Operation

• Class II A1
• not designed for chemical use
• May be used for non-volatile toxic chemicals or
  low-level radioactive materials
• Class II A2
• may be used for minute amounts of volatile
  chemicals if canopy connected
• Ensure annual certification
• Place all work materials into cabinet before
• starting

15
Biological Safety Cabinet Operation

• CAUTIONS
• Chemicals may damage HEPA filter
• Volatile chemicals NOT retained by HEPA filter
• Exposes personnel if not exhausted
• Fans NOT spark proof
• Chemical use may result in fire and/or explosion
• Never use flammables

16
Centrifuges
17
Centrifuge - Hazards

• Mechanical failure
• Lab equipment failure
• tubes etc.
• Aerosol generation
• Operator error

18
Centrifuge - Operation

• 1. Check tubes for cracks/chips
• Use matched sets of tubes, buckets etc
• Make sure the rotor is balanced
• Tightly seal all tubes and safety cups
• Ensure that rotor is locked to spindle
• and the bucket is seated
• 5. Close lid during operation
• Allow to come to complete stop before
• opening

19
Centrifuge - Operation

• Use safety cups whenever possible
• Sealed rotors required when processing RG-3
  agents
• Disinfect weekly and after all spills or
• breaks
• Lubricate O-rings and rotor threads
• weekly
• Do not use rotors that have been dropped
• Contact centrifuge representative
• When specific information is needed

20
This completes Module 1C

• Click on the X at the top of the page to close
  this window. Then
• Continue on with Module 1D or
• Comment or ask questions about Module 1C by
  clicking on the link on the main page of the
  course