Biosafety and Biohazardous Materials: An Introduction

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Biosafety and Biohazardous MaterialsAn
Introduction
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Biosafety at ISU

• Biosafety
• The application of knowledge, techniques and
  equipment to prevent personal, laboratory and
  environmental exposure to potentially infectious
  agents or biohazards.
• Biosafety Officer
• The Biosafety Officer for Iowa State University
  is Dr. Betsy Matos. To contact Betsy with a
  question call 294-5359.

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Bloodborne Pathogen

• Anyone working with human blood or other
  infectious agents including mammalian cell lines
  must be enrolled in the bloodborne pathogen
  program and take bloodborne pathogen training
  annually.
• Details about the program can be found in the
  Bloodborne Pathogens manual which is on the EHS
  website.

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Biohazardous Waste Oversight

• All biohazardous waste should be decontaminated
  before disposal.
• Be sure to place an X over the biohazard symbol
  on biohazard bags before placing them in the
  autoclave.

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Waste Disposal

• All biohazardous waste must be disposed of
  according to the ISU Sharps and Biohazardous
  Waste Policy.
• Decontamination or disinfection

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Sharps Safety

• Dispose of sharps in a puncture resistant
  container.
• Do not throw sharps, including plastic pipettes,
  into the trash.

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Biosafety Cabinets (BSCs)

• EHS coordinates the annual certification of
  biosafety cabinets.
• All BSCs used with infectious substances must be
  certified annually.
• EHS offers training on how to properly use BSCs.

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Biosafety Cabinets, Laminar Flow Hoods, and Fume
Hoods
Clean Bench
Biosafety Cabinet
Fume Hood
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Autoclave Validation

• EHS provides autoclave validation testing (spore
  vials).
• EHS offers training on how to safely and
  effectively use autoclaves.

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Biological Materials Inventory

• A resource for first responders (think firemen)
  to identify the location of potentially
  infectious materials in an emergency.
• Online database with intuitive data entry tools.
  Call EHS 294-5359.

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Laboratory Equipment Disposal

• Disposed of through ISU Asset Recovery.
• All laboratory equipment must be inspected and
  approved for disposal by EHS, call 294-5359.

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Biohazardous Materials

• Definition- Materials of biological origin that
  could potentially cause harm to humans, domestic
  or wild animals, plants or the environment.

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Examples

• Transgenic animals or plants
• Human, animal or plant pathogens
• Biological toxins (such as botulinum toxin)
• Human blood and certain human body fluids
• Human or non-human primate cell cultures

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BioterrorismPotential

• Bioterrorism use or biological warfare
• Bacteria i.e. Anthrax
• Agroterrorism
• Plant
• Livestock

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Regulations

• Regulations and requirements depend on the type
  of biological agent used in the research.

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USDA
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ISU Review Committees

• Institutional Review Board (IRB), Human Subjects
  Committee
• Institutional Biosafety Committee (IBC)
• Institutional Animal Care and Use Committee
  (IACUC)
• Radiation Safety Committee (RSC)

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Biosafety Level Guidelines

• CDC and NIH guidelines ensure proper work
  practices.
• ISU Biosafety
• Manual is a safety resource.

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Medical Surveillance

• Hazard Inventory
• Vaccinations and testing
• What to do if exposed.

View of inventory form
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Points of Contact

• Betsy Matos, Anne Dombroski, Amy Helgerson, Steve
  Ziegenfuss.

EHS 294-5359