CenterSpecific Neuropathology Procedures Survey

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Center-Specific Neuropathology Procedures Survey

• Erin M. Pfeiffer
• 10/12/2002

NACC
National Alzheimers Coordinating Center
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Center-Specific Neuropathology Survey

• Dr. Cochran, Dr. Dickson, Dr. Ghetti
• Special Thanks Dr. Jim Leverenz
• 10-page detailed survey
• 90.6 response rate (29/32 surveys)
• Complete answers thoughtful comments

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Survey Form
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Survey Form
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Survey Form
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Survey Form
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General Questions NP-related data elements
maintained in each Centers Database
Frequency Missing 1
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General Questions (contd)

• gt 90 maintaining data on
• AD pathological dx
• Plaques/tangles
• PD pathological dx
• Lewy bodies
• Infarcts
• 46 maintain genetic data
• 62 collect circumstance of death
• (Including Centers who collect for some/not
  all
• subjects)

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Specimen and Tissue PreparationPostmortem
Interval
of Centers
Postmortem Interval (hrs)
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Specimen and Tissue PreparationSpecimens
Collected from Autopsy
PNT Peripheral Nervous Tissue
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Specimen and Tissue PreparationCryoprotection

• 40 store fixed tissue in cryoprotective
  solution
• Average time tissue fixed prior to transfer to
  cryoprotective solution 2 days
• Cryoprotective solutions include
• 16 sucrose
• 20 glycerol, 2 DMSO
• Isopentane Liquid Nitrogen
• Sucrose/ethylene glycol in phosphate buffer

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Specimen and Tissue PreparationBiological
Specimen Banking
ICL Immortalized Cell Lines
Frequency Missing 2
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HistopathologyStains

• Stains for identifying beta-amyloid pathology
• 69 Silver Stain (e.g. Bielschowsky, Bodian,
• Campbell-Switzer, Microwave King,
  Gallyas)
• 35 Thioflavin-S
• 24 Congo Red
• 72 Aß immunostain (e.g. GFAP, Novocastra,
• DAKO monoclonal mouse)

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HistopathologyStains

• Stains for identifying neurofibrillary pathology
• 76 Silver Stain (e.g. Bielschowsky,
  Modified-Biel,
• Bodian, Microwave King, Gallyas)
• 36 Thioflavin-S
• 83 Tau immunostain (e.g. AT8, PHF-1, ALZ-50,
• Tau-2)

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NeuropathologyCriteria
Washington U modified Koch (1985), In house
semi-quantitative methods, etc
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NeuropathologyCriteria
Frequency Missing 1
Lund and Manchester Groups. Clinical and
neuropathological criteria for frontotemporal
dementia. J of Neurology, Neurosurgery, and
Neuropsychiatry 1994416-18 McKhann GM et al.
Clinical and Pathological Diagnosis of
Frontotemporal Dementia report of the work
group on frontotemporal dementia and Picks
disease. Arch of Neurol 20011803-09
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Additional ProceduresPerformed on NP core tissue

• Percentage of Centers that perform
• Immunoassays 40
• In situ hybridization 14
• Image analysis 36
• Stereology 25
• Microarray analysis 32

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Summary / Plans for NP Survey Data

• Detailed summary of NP procedures specific
• specimens available at each ADC
• Dataset created - After NYC, contact a few ADCs
  
• for brief clarifications
• Survey results will be available via NACCs
  website
• Data will be integrated into overall database
• (i.e. linked to MDS, Neuropathology data,
  other
• Centers data)
• Thanks again!

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