Manipulating the cycle to synchronize

1
Manipulating the cycle to synchronize

• Mice- hCG
• Cattle- 2 shots PGF2a or P4implant
• Equine- reference heat with PGF2a

2
Manipulating the Ovary- Superovulation

• Mice PMS/hCG
• Cattle FSH
• Equine NOT
• Sheep/goat FSH
• Human - FSH

3
Manipulating the Egg in vitro maturation

• LH and FSH
• E2
• 24 hours
• 75 mature

4
Cleavage(2-cell to morula)

• Cell increases
• Cell size decreases
• Cells are totipotent
• Not synchronous
• Energy source pyruvate and lactate

5
Blastocyst

• Blastoceole forms
• Trophoblast/ICM forms
• Tight junctions form
• Gap junctions form
• Na pump forms
• Energy source glucose
• Embryo size increases

6
Hatching

• Zona stretches
• Broken down by proteolytic enzymes from TB cells
• In vitro zona hardening

7
Assisted Hatching

• Micromanipulation procedure
• Acid tyrodes dissolves portion of zona
• Releases embryo from hardened zona

8
Embryo Assessment Based Upon

• Blastomere quality
• Cytoplasmic anomalies
• Rate of cleavage

9
Symmetrical vs Asymmetrical
10
Multinucleation
11
Granularity
12
Fragmentation
13
Vacuoles
14
Deformities
15
Thickened Zona